CPA anticorps (Biotin)
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- Antigène Tous les produits CPA
- CPA (Carboxypeptidase A (CPA))
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Reactivité
- Boeuf (Vache)
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp CPA est conjugé à/à la Biotin
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Application
- Western Blotting (WB), ELISA
- Réactivité croisée (Details)
- Cross reactivity against Carboxypeptidase A from other sources may occur but have not been specifically determined.
- Pureté
- Anti-Carboxypeptidase A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase A [Bovine Pancreas].
- niveau d'endotoxine
- Low Endotoxin : No
- Immunogène
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Carboxypeptidase A [Bovine Pancreas]
Immunogen Type: Native Protein - Isotype
- IgG
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- Indications d'application
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Anti-Carboxypeptidase A has been assayed against 1.0 µg of Carboxypeptidase A in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:15.000 to 1:55.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 10 mg/mL
- Buffer
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free - Conseil sur la manipulation
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- Stock
- -20 °C
- Stockage commentaire
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- Date de péremption
- Expiration date is one (1) year from date of opening.
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- Antigène
- CPA (Carboxypeptidase A (CPA))
- Autre désignation
- Carboxypeptidase A (CPA Produits)
- Sujet
- Anti-Carboxypeptidase A antibody catalyzes the release of C-terminal amino acids with little or no action with –Asp, -Glu, -Arg, -Lys or –Pro. This antibody is ideal for investigators interested in Metabolism, Cancer, Signal Transduction, or Cell Biology research. Synonyms: Carboxypeptidase A1 precursor antibody, CPA1 antibody, Pancreatic carboxypeptidase A1 antibody, Procarboxypeptidase A1 pancreatic antibody
- ID gène
- 286762
- UniProt
- P00730
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