Malate Dehydrogenase (MDH) anticorps (Biotin)

N° du produit ABIN1607839

Détail du produit

Antigène: Malate Dehydrogenase (MDH)

Reactivité: Porc

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Biotin

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Réactivité croisée (Details): Cross reactivity against Malate Dehydrogenase from other sources is unknown.

Pureté: Anti-Malate dehydrogenase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Malate Dehydrogenase [Pig Heart]
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-Malate dehydrogenase antibody has been assayed against 1.0 µg of Malate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3.000 to 1:11.000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 µg of Malate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3.000 to 1:11.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détail du antigène

Antigène: Malate Dehydrogenase (MDH)

Autre désignation: Malate Dehydrogenase (MDH Produits)

Synonymes: anticorps CG5889, anticorps Dmel\\CG5889, anticorps MDH, anticorps Mdh, anticorps anon-EST:Posey79, anticorps MALATE DEHYDROGENASE, anticorps malate dehydrogenase, anticorps An15g00070, anticorps AO090701000013, anticorps DDBDRAFT_0188779, anticorps DDBDRAFT_0230186, anticorps DDB_0188779, anticorps DDB_0230186, anticorps Malic enzyme b, anticorps oxidoreductase, putative, anticorps malate dehydrogenase, anticorps NAD dependent malate dehydrogenase Mdh, anticorps Men-b, anticorps malate dehydrogenase, anticorps MDH, anticorps mdh, anticorps APH_RS02635, anticorps ANI_1_12134, anticorps AOR_1_26114, anticorps PMI_RS16925, anticorps mdhA, anticorps mdh1

Sujet: Anti-Malate dehydrogenase recognizes the protein malate dehydrogenase, an oxidoreductase that catalyzes the oxidation of malate to oxaloacetate with the concomitant reduction of NAD+ to NADH. This reaction occurs in various metabolic pathways, the most notable being the citric acid cycle and gluconeogenesis. Two isoforms of malate dehyrogenase exist in eukaryotes localized in either the cytoplasm or the mitochondrial matrix. Synonyms: Malate dehydrogenase, cytoplasmic EC=1.1.1.37 Cytosolic malate dehydrogenase

ID gène: 396894

UniProt: P11708

Pathways: Regulation of Lipid Metabolism by PPARalpha