Malate Dehydrogenase (MDH) anticorps (HRP)

N° du produit ABIN5596953

Détail du produit

Antigène: Malate Dehydrogenase (MDH)

Reactivité: Porc

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Réactivité croisée (Details): Cross reactivity against Malate Dehydrogenase from other sources may occur but has not been specifically determined.

Purification: Malate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart].

Immunogène:

Immunogen: Malate Dehydrogenase [Pig Heart]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: Anti-Malate Dehydrogenase is useful in ELISA, western blot, and immunohistochemistry. Optimal titers should be obtained by researchers.

Western Blot Dilution: 1:1,000 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:10,000 - 1:50,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détail du antigène

Antigène: Malate Dehydrogenase (MDH)

Autre désignation: Malate Dehydrogenase (MDH Produits)

Synonymes: anticorps CG5889, anticorps Dmel\\CG5889, anticorps MDH, anticorps Mdh, anticorps anon-EST:Posey79, anticorps MALATE DEHYDROGENASE, anticorps malate dehydrogenase, anticorps An15g00070, anticorps AO090701000013, anticorps DDBDRAFT_0188779, anticorps DDBDRAFT_0230186, anticorps DDB_0188779, anticorps DDB_0230186, anticorps Malic enzyme b, anticorps oxidoreductase, putative, anticorps malate dehydrogenase, anticorps NAD dependent malate dehydrogenase Mdh, anticorps Men-b, anticorps malate dehydrogenase, anticorps MDH, anticorps mdh, anticorps APH_RS02635, anticorps ANI_1_12134, anticorps AOR_1_26114, anticorps PMI_RS16925, anticorps mdhA, anticorps mdh1

Sujet:

Synonyms: Malate dehydrogenase, cytoplasmic EC=1.1.1.37 Cytosolic malate dehydrogenase

Background: Malate Dehydrogenase is an enzyme that reversibly catalyzes the oxidation of malate to oxaloacetate using the reduction of NAD+ to NADH. Malate Dehydrogenase is involved in gluconeogenesis, the synthesis of glucose from smaller molecules. There are two main isoforms in eukaryotic cells. One is found in the mitochondrial matrix the other is found in the cytoplasm.

Gene Name: MDH1

ID gène: 396894

UniProt: P11708

Pathways: Regulation of Lipid Metabolism by PPARalpha