Human Papilloma Virus 16 E6 (HPV-16 E6) (AA 85-158) anticorps (Alexa Fluor 350)

N° du produit ABIN1692573

Détail du produit

Antigène: Human Papilloma Virus 16 E6 (HPV-16 E6)

Épitope: AA 85-158

Reactivité: Human Papillomavirus 16 (HPV-16), Virus

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Alexa Fluor 350

Application: Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Réactivité croisée: Virus

Réactivité croisée (Details): HPV16

Purification: Purified by Protein G.

Immunogène: KLH conjugated synthetic peptide derived from HPV16 E6 protein

Clone: 2E3

Isotype: IgG

Information d'application

Indications d'application: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: Human Papilloma Virus 16 E6 (HPV-16 E6)

Autre désignation: HPV16 E6 protein

Classe de substances: Viral Protein

Sujet:

Synonyms: Protein E6, E6

Background: Plays a major role in the induction and maintenance of cellular transformation. Acts mainly as an oncoprotein by stimulating the destruction of many host cell key regulatory proteins. E6 associates with host E6-AP ubiquitin-protein ligase, and inactivates tumor suppressors TP53 and TP73 by targeting them to the 26S proteasome for degradation. In turn, DNA damage and chromosomal instabilities increase and lead to cell proliferation and cancer development. The complex E6/E6P targets several other substrates to degradation via the proteasome including host NFX1-91, a repressor of human telomerase reverse transcriptase (hTERT). The resulting increased expression of hTERT prevents the shortening of telomere length leading to cell immortalization. Other cellular targets including Bak, Fas-associated death domain-containing protein (FADD) and procaspase 8, are degraded by E6/E6AP causing inhibition of apoptosis. E6 also inhibits immune response by interacting with host IRF3 and TYK2. These interactions prevent IRF3 transcriptional activities and inhibit TYK2-mediated JAK-STAT activation by interferon alpha resulting in inhibition of the interferon signaling pathway.

ID gène: 1489078

UniProt: P03126