PTPRF anticorps (AA 1315-1607) (Atto 488)

N° du produit ABIN1740983

Détail du produit anti-PTPRF anticorps (Atto 488)

Antigène: PTPRF (Protein Tyrosine Phosphatase Receptor Type F (PTPRF))

Épitope: AA 1315-1607

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PTPRF est conjugé à/à la Atto 488

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificité: Detects ~85 kDa (full length protein is 210 kDa - smaller due to proteolysis into P-subunit containing transmembrane and intracellular domains.

Réactivité croisée: Humain, Souris, Rat

Purification: Protein G Purified

Immunogène: Fusion protein amino acids 1315-1607 (cytoplasmic C-terminus) of human LAR. 97% identical in both rat and mouse. >80% identity with PTPRD and PTPRS. >50% identity with PTPRM and PTPRK.

Clone: S165-38

Isotype: IgG2a

Information d'application

Indications d'application:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg/ml of ABIN1740983 was sufficient for detection of LAR/PTPRF in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur PTPRF

Antigène: PTPRF (Protein Tyrosine Phosphatase Receptor Type F (PTPRF))

Autre désignation: LAR (PTPRF Produits)

Synonymes: anticorps LAR, anticorps AA591035, anticorps LARS, anticorps RPTP-LAR, anticorps LARFN5C, anticorps zf-LAR, anticorps protein tyrosine phosphatase, receptor type F, anticorps protein tyrosine phosphatase, receptor type, F, anticorps protein tyrosine phosphatase, receptor type, f, a, anticorps PTPRF, anticorps Ptprf, anticorps ptprfa

Sujet: PTPRF or leukocyte common antigen-related protein (LAR) is a widely expressed protein tyrosine phosphatase with an extracellular receptor region that resembles a cell adhesion molecule. PTPRF removes phosphate group from β-catenin, an event that may subsequently facilitate cell-cell adhesion and ensure the stability of the cadherin complex. This phosphatase has also been implicated in various cellular processes such as neurite growth, nerve regeneration, actin remodeling and regulation of insulin function (1,2,3,4). Anti-PTPRF (C-terminal) antibody is specific for the extracellular and cytoplasmic subunits of human PTPRF (approx. 210, 150 and 85 kDa). Detection of the PTPRF bands by immunoblotting is specifically inhibited by the immunizing peptide.

ID gène: 5792

UniProt: P10586

Pathways: EGFR Signaling Pathway