VAMP1, 2, 3 (AA 1-81) anticorps
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- Antigène
- VAMP1, 2, 3
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Épitope
- AA 1-81
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Reactivité
- Souris, Rat, Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Inconjugué
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Application
- Western Blotting (WB), Immunocytochemistry (ICC)
- Specificité
- Specific for all three isoforms VAMP 1, 2 and 3.
- Purification
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
- Immunogène
- Recombinant protein of the cytoplasmic part of rat cellubrevin (aa 1-81).
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- Indications d'application
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WB: 1 : 1000 up to 1 : 5000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 200 - Commentaires
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ICC: Methanol and acetone fixation, see Annaert et al., 1997 This antibody is recommended for ICC of non-neuronal cells. This antibody detects the tetanus and Botulinum B toxin cleavage product in toxin treated tissue homogenates.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS
- Conseil sur la manipulation
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- Stock
- -20 °C
- Stockage commentaire
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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Botulinum protease-cleaved SNARE fragments induce cytotoxicity in neuroblastoma cells." dans: Journal of neurochemistry, Vol. 129, Issue 5, pp. 781-91, (2014) (PubMed).
: "
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Botulinum protease-cleaved SNARE fragments induce cytotoxicity in neuroblastoma cells." dans: Journal of neurochemistry, Vol. 129, Issue 5, pp. 781-91, (2014) (PubMed).
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- Antigène
- VAMP1, 2, 3
- Sujet
- Synonyms: VAMP 3
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