SYT2 anticorps (Cytoplasmic Domain)

N° du produit ABIN1742202

Détail du produit anti-SYT2 anticorps

Antigène: SYT2 (Synaptotagmin II (SYT2))

Épitope: AA 406-422, Cytoplasmic Domain

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SYT2 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Specificité: Specific for synaptotagmin 2.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogène: Synthetic peptide (aa 406-422 in rat synaptotagmin 2) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Information d'application

Indications d'application: WB: 1 : 1000 (AP staining)
ICC: not tested yet
IHC: 1 : 500 up to 1 : 1000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Conseil sur la manipulation: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Stock: -20 °C

Stockage commentaire: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Références pour anticorps anti-SYT2 (ABIN1742202)

Whitemarsh, Strathman, Chase, Stankewicz, Tepp, Johnson, Pellett: "Novel application of human neurons derived from induced pluripotent stem cells for highly sensitive botulinum neurotoxin detection." dans: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 126, Issue 2, pp. 426-35, (2012) (PubMed).

Culver, Savas, Park, Choi, Zheng, Zeitlin, Yates, Tanese: "Proteomic analysis of wild-type and mutant huntingtin-associated proteins in mouse brains identifies unique interactions and involvement in protein synthesis." dans: The Journal of biological chemistry, Vol. 287, Issue 26, pp. 21599-614, (2012) (PubMed).

Johnson, Franz, Kuhn, Furness, Rüttiger, Münkner, Rivolta, Seward, Herschman, Engel, Knipper, Marcotti: "Synaptotagmin IV determines the linear Ca2+ dependence of vesicle fusion at auditory ribbon synapses." dans: Nature neuroscience, Vol. 13, Issue 1, pp. 45-52, (2009) (PubMed).

Détails sur SYT2

Antigène: SYT2 (Synaptotagmin II (SYT2))

Autre désignation: Synaptotagmin 2 (SYT2 Produits)

Synonymes: anticorps SytII, anticorps RATSYNII, anticorps SYNII, anticorps R74640, anticorps mKIAA4194, anticorps si:ch211-236j4.1, anticorps synaptotagmin 2, anticorps synaptotagmin II, anticorps synaptotagmin IIa, anticorps SYT2, anticorps Syt2, anticorps syt2a, anticorps syt2

Pathways: Synaptic Vesicle Exocytosis