Proton ATPase (AA 826-838) anticorps

N° du produit ABIN1742216

Détail du produit

Antigène: Proton ATPase

Épitope: AA 826-838

Reactivité: Hamster, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Specificité: Specific for the alpha1 116kDa subunit.

Purification: antiserum

Immunogène: Synthetic peptide FSFEHIREGKFDE (826-838 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Information d'application

Indications d'application: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 100
IHC-P: 1 : 100

Commentaires:

WB: The proton pump aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples. ICC: Methanol fixation is recommended.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS

Conseil sur la manipulation: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Stock: 4 °C/-20 °C

Stockage commentaire: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Référence

Gouzy, Larrouy-Maumus, Bottai, Levillain, Dumas, Wallach, Caire-Brandli, de Chastellier, Wu, Poincloux, Brosch, Guerquin-Kern, Schnappinger, Sório de Carvalho, Poquet, Neyrolles: "Mycobacterium tuberculosis exploits asparagine to assimilate nitrogen and resist acid stress during infection." dans: PLoS pathogens, Vol. 10, Issue 2, pp. e1003928, (2014) (PubMed).

Yao, de la Iglesia, Bajjalieh: "Loss of the SV2-like protein SVOP produces no apparent deficits in laboratory mice." dans: PLoS ONE, Vol. 8, Issue 7, pp. e68215, (2013) (PubMed).

Détail du antigène

Antigène: Proton ATPase