ARC (AA 1-214) anticorps

N° du produit ABIN1742333

Détail du produit

Antigène: ARC

Épitope: AA 1-214

Reactivité: Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: ELISA, Immunoprecipitation (IP)

Specificité: Specific for Arc.

Purification: purified IgG. Azide was added before lyophilization.

Immunogène: Strep-Tag® fusion protein of full-length mouse arc. Epitope Binds within first 214 aa.

Clone: 48B2

Isotype: IgG2b

Information d'application

Indications d'application: WB: not recommended
ICC: not tested yet
IHC: not tested yet

Commentaires:

ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Stock: -20 °C

Stockage commentaire: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Référence

Izumi, Ishimoto, Yamamoto, Mori: "Application of hairless mouse strain to bioluminescence imaging of Arc expression in mouse brain." dans: BMC neuroscience, Vol. 18, Issue 1, pp. 18, (2017) (PubMed).

Gerace, Landucci, Totti, Bani, Guasti, Baronti, Moroni, Mannaioni, Pellegrini-Giampietro: "Ethanol Toxicity During Brain Development: Alterations of Excitatory Synaptic Transmission in Immature Organotypic Hippocampal Slice Cultures." dans: Alcoholism, clinical and experimental research, Vol. 40, Issue 4, pp. 706-16, (2016) (PubMed).

Lee, Kang, Kim, Yoon, Kim, Moon: "Enhanced expression of immediate-early genes in mouse hippocampus after trimethyltin treatment." dans: Acta histochemica, (2016) (PubMed).

Alvarez-Dieppa, Griffin, Cavalier, McIntyre: "Vagus Nerve Stimulation Enhances Extinction of Conditioned Fear in Rats and Modulates Arc Protein, CaMKII, and GluN2B-Containing NMDA Receptors in the Basolateral Amygdala." dans: Neural plasticity, Vol. 2016, pp. 4273280, (2016) (PubMed).

Vasileva, Horstmann, Geumann, Gitler, Kuner: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." dans: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).

Détail du antigène

Antigène: ARC

Synonymes: anticorps activity regulated cytoskeleton associated protein, anticorps Arc

Sujet: Synonyms: Arg 3.1