Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
Specificité
Detects preferentially Doc 2a
Réactivité croisée (Details)
some cross-reactivity to Doc 2b.
Purification
Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Immunogène
Recombinant full length rat Doc 2a protein.
Indications d'application
WB: 1 : 1000 IP: not tested yet ICC: 1 : 100 up to 1 : 500 IHC: 1 : 200
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer
PBS
Conseil sur la manipulation
Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
Stock
-20 °C
Stockage commentaire
Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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Schütz, Zilly, Lang, Jahn, Bruns: "A dual function for Munc-18 in exocytosis of PC12 cells." dans: The European journal of neuroscience, Vol. 21, Issue 9, pp. 2419-32, (2005) (PubMed).