GABRA1 anticorps (AA 28-43)

N° du produit ABIN1742456

Détail du produit anti-GABRA1 anticorps

Antigène: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Épitope: AA 28-43

Reactivité: Rat, Souris

Hôte: Cobaye

Clonalité: Polyclonal

Conjugué: Cet anticorp GABRA1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificité: Specific for GABA-A receptor alpha1.

Purification: antiserum

Immunogène: Synthetic peptide (aa 28-43 of rat GABA-A receptor alpha1 precursor protein) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.

Information d'application

Indications d'application: WB: 1 : 1000 up to 1 : 2000 (AP staining)
IP: not tested yet
ICC: 1 : 200 up to 1 : 1000
IHC: 1 : 500

Commentaires:

WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ICC: This antibody is PFA fixation sensitive, use only mild fixation (2% PFA). Best results are obtained by application on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents. IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS

Conseil sur la manipulation: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Stock: 4 °C/-20 °C

Stockage commentaire: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Détails sur GABRA1

Antigène: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Autre désignation: GABA-A Receptor Alpha1 (GABRA1 Produits)

Synonymes: anticorps ECA4, anticorps EJM, anticorps EJM5, anticorps zgc:153566, anticorps Gabra-1, anticorps gamma-aminobutyric acid type A receptor alpha1 subunit, anticorps gamma-aminobutyric acid (GABA) A receptor, alpha 1, anticorps gamma-aminobutyric acid (GABA) A receptor, subunit alpha 1, anticorps gamma-aminobutyric acid receptor subunit alpha-1, anticorps GABRA1, anticorps Gabra1, anticorps gabra1, anticorps LOC100653509

Sujet: Synonyms: GABA(A) R alpha1