Glu-alpha-Tubulin (C-Term) anticorps

N° du produit ABIN1742545

Détail du produit

Antigène: Glu-alpha-Tubulin

Épitope: C-Term

Reactivité: Humain, Souris, Rat, Eucaryotes, Poisson zèbre (Danio rerio)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificité: Specific for detyrosinated alpha-tubulin (glu-tubulin) and polyglutamylated tubulin (also beta-tubulin).

Réactivité croisée (Details): No cross reaction to tyrosinated tubulin.

Purification: purified IgG. Azide was added before lyophilization.

Immunogène: Synthetic peptide VDSVEGEGEEEGEE (14 C-terminal aa of detyrosinated alpha-tubulin) coupled to BSA via glutaraldehyde. Epitope X-EE including the terminal alpha-carboxylate group. All linkages in the peptide involve alpha-peptide bonds.

Clone: 1D5

Isotype: IgG1

Information d'application

Indications d'application: WB: 1 : 100 up to 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 200
IHC: 1 : 200

Commentaires:

WB: Tween 20 (other detergents not yet tested) should not be added to the blocking and washing solution. It greatly diminishes the intensity of the signal.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Stock: -20 °C

Stockage commentaire: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Référence

Zink, Grosse, Freikamp, Bänfer, Müksch, Jacob: "Tubulin detyrosination promotes monolayer formation and apical trafficking in epithelial cells." dans: Journal of cell science, Vol. 125, Issue Pt 24, pp. 5998-6008, (2013) (PubMed).

Jablonka, Dombert, Asan, Sendtner: "Mechanisms for axon maintenance and plasticity in motoneurons: alterations in motoneuron disease." dans: Journal of anatomy, Vol. 224, Issue 1, pp. 3-14, (2013) (PubMed).

Roll-Mecak, Vale: "Structural basis of microtubule severing by the hereditary spastic paraplegia protein spastin." dans: Nature, Vol. 451, Issue 7176, pp. 363-7, (2008) (PubMed).

Zhang, Gilquin, Khochbin, Matthias: "Two catalytic domains are required for protein deacetylation." dans: The Journal of biological chemistry, Vol. 281, Issue 5, pp. 2401-4, (2006) (PubMed).

Rosales-Nieves, Johndrow, Keller, Magie, Pinto-Santini, Parkhurst: "Coordination of microtubule and microfilament dynamics by Drosophila Rho1, Spire and Cappuccino." dans: Nature cell biology, Vol. 8, Issue 4, pp. 367-76, (2006) (PubMed).

Yoon, Shin, Mercurio: "Hypoxia stimulates carcinoma invasion by stabilizing microtubules and promoting the Rab11 trafficking of the alpha6beta4 integrin." dans: Cancer research, Vol. 65, Issue 7, pp. 2761-9, (2005) (PubMed).

Van der Heiden, Groenendijk, Hierck, Hogers, Koerten, Mommaas, Gittenberger-de Groot, Poelmann: "Monocilia on chicken embryonic endocardium in low shear stress areas." dans: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 235, Issue 1, pp. 19-28, (2005) (PubMed).

Rüdiger, Rüdiger, Wehland, Weber: "Monoclonal antibody ID5: epitope characterization and minimal requirements for the recognition of polyglutamylated alpha- and beta-tubulin." dans: European journal of cell biology, Vol. 78, Issue 1, pp. 15-20, (1999) (PubMed).

Wheatley, Feilen, Yin, Wheatley: "Primary cilia in cultured mammalian cells: detection with an antibody against detyrosinated alpha-tubulin (ID5) and by electron microscopy." dans: Journal of submicroscopic cytology and pathology, Vol. 26, Issue 1, pp. 91-102, (1994) (PubMed).

Détail du antigène

Antigène: Glu-alpha-Tubulin