ELISA: Indirect: To detect hAITRL (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hAITRL. Sandwich: To detect hAITRL using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with Biotinylated Anti-HumanAITRL as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hAITRL. Western blot: To detect hAITRL this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hAITRL is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.
Buffer
PBS, pH 7.2
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
-20 °C
Stockage commentaire
Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
Date de péremption
6 months
Antigène
TNFSF18
(Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18))