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E-cadherin anticorps (AA 375-631)

CDH1 Reactivité: Porc WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1858334
  • Antigène Voir toutes E-cadherin (CDH1) Anticorps
    E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
    Épitope
    • 17
    • 17
    • 16
    • 16
    • 16
    • 15
    • 8
    • 8
    • 7
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 375-631
    Reactivité
    • 251
    • 103
    • 87
    • 18
    • 16
    • 16
    • 9
    • 9
    • 9
    • 6
    • 4
    • 4
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Porc
    Hôte
    • 201
    • 83
    • 8
    • 7
    Lapin
    Clonalité
    • 187
    • 111
    • 1
    Polyclonal
    Conjugué
    • 140
    • 36
    • 29
    • 10
    • 10
    • 9
    • 8
    • 8
    • 7
    • 6
    • 6
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp E-cadherin est non-conjugé
    Application
    • 206
    • 94
    • 91
    • 68
    • 56
    • 56
    • 39
    • 34
    • 29
    • 27
    • 17
    • 16
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Fonction
    Polyclonal Antibody to E-cadherin
    Specificité
    The antibody is a rabbit polyclonal antibody raised against E-cadherin. It has been selected for its ability to recognize E-cadherin in immunohistochemical staining and western blotting.
    Purification
    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
    Immunogène
    Recombinant E-cadherin corresdonding to Phe375~Thr631 (Accession # C6EVT4) with N-terminal His and GST Tag
    Isotype
    IgG
    Top Product
    Discover our top product CDH1 Anticorps primaire
  • Indications d'application

    Western blotting: 0.5-2 μg/mL

    1:160-660 Immunohistochemistry: 5-20 μg/mL

    1:16-66 Immunocytochemistry: 5-20 μg/mL

    1:16-66 Optimal working dilutions must be determined by end user.

    Commentaires

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions
    For Research Use only
  • Validation #101345 (Immunofluorescence)
    'Independent Validation' signe
    by
    Sackler School of Biomedical Sciences, Tufts University School of Medicine
    No.
    #101345
    Date
    05.06.2017
    Antigène
    E-cadherin
    Numéro du lot
    A20160427539
    Application validée
    Immunofluorescence
    Contrôle positif
    porcine unerupted tooth buds, dental epithelium
    Contrôle négative
    porcine unerupted tooth buds, mesenchym
    Conclusion
    Passed. The E-caherin antibody ABIN1858334 specifically labels epithelial tissues in porcine unerupted tooth buds in immunofluorescence, consistent with the expression pattern of E-cadherin.
    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    ABIN1858334
    Anticorps secondaire
    goat anti-rabbit AF568 conjugated antibody (ThermoFisher Scientific, A11011, lot 1778025)
    Full Protocol
    • Harvest unerupted tooth buds from 5 month old pig jaws and wash 3x in phosphate bufferend saline (PBS) (Boston Bioproducts, BM-220).
    • Fix tooth buds in 10% Formalin (Fisher Scientific, 23-245-685) for 24h.
    • Wash 3x in PBS.
    • Place tooth buds through graded ethanol seris for 6h at each concentration: 50%, 70%, 80%, 90%, 100%, 100%, 100%.
    • Place tooth buds in 3x 2h in 100% xylene.
    • Place in molten paraffin 2x (TissuePrep 2 Paraffin, Fisher, T555-2) 12h.
    • Embed tooth buds in paraffin and cool on a 4°C cold plate for 1h.
    • Let set ON at RT.
    • Cut blocks into 6µm sections using a microtome (ThermoFisher Scientific, HM 355S).
    • Use a tissue floating bath to mount sections onto microscope slides.
    • Heat sections on a hot plate for 1h at 45°C for 1h then ON at 55°C.
    • Deparaffinize slide 2x 5 min in 100% xylene.
    • Rehydrate sections in graded ethanol series for 2min for each concentration: 100%, 95%, 70%, 50%.
    • Place slide in H2O for 5min.
    • Transfer slide to a plastic holder filled with warmed 10mM Citric Acid Solution (made from sodium citrate monobasic; Sigma, 71497) pH6.0 and incubate in a steamer for 20min.
    • Cool submerged slide on benchtop for 30min.
    • Wash slide 3x 5min in PBS.
    • Create a hydrophobic barrier on slide around sections with PAP-pen (Vector Labs, H-4000, lot 5138).
    • Place slide in a humidified chamber and block with 5% BSA for 20min at 37°C.
    • Gently remove BSA by tapping slide.
    • Incubate sections with primary antibody
      • rabbit anti-pig E-cadherin antibody (antibodies-online, ABIN1858334, lot A20160408626) diluted to 1:40 in 1% BSA or
      • and mouse anti-pig Vimentin (Santa Cruz Biotechnology, SC-6260, lot G2814) diluted to 1:500 in 1% BSA for 1h at RT.
    • A no primary antibody negative control was incubated in parallel in 1% BSA.
    • Wash in slide 3x 5min in PBS.
    • Incubate sections in secondary antibodies
      • goat anti-rabbit AF568 conjugated antibody (ThermoFisher Scientific, A11011, lot 1778025) diluted to 1:100 in 1% BSA and
      • goat anti-mouse AF488 conjugated antibody (ThermoFisher Scientific, A11001, lot 1752514 ) diluted to 1:100 in 1% BSA for 1h at RT.
    • Wash in slide 3x 5min in PBS.
    • Add approximately 10μL of Hard Set Mounting Medium with DAPI (Vector Labs, H-1500, lot ZC1221) for each section and mount cover slip.
    Notes
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    Date de péremption
    24 months
  • Smith, Zhang, Schiele, Khademhosseini, Kuo, Yelick: "Developing a biomimetic tooth bud model." dans: Journal of tissue engineering and regenerative medicine, (2017) (PubMed).

    Monteiro, Smith, Angstadt, Zhang, Khademhosseini, Yelick: "Dental cell sheet biomimetic tooth bud model." dans: Biomaterials, Vol. 106, pp. 167-79, (2016) (PubMed).

  • Antigène
    E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
    Autre désignation
    E-cadherin (CDH1 Produits)
    Synonymes
    anticorps Arc-1, anticorps CD324, anticorps CDHE, anticorps ECAD, anticorps LCAM, anticorps UVO, anticorps E-cadherin, anticorps cdh1, anticorps si:dz180o5.2, anticorps uvo, anticorps lcam, anticorps E-Cad, anticorps XBcad, anticorps l-cam, anticorps xcdh1, anticorps cdhc-A, anticorps xb-cad, anticorps XTCAD-1, anticorps c-cadherin, anticorps cadherin-1, anticorps uvomorulin, anticorps XB-cadherin, anticorps arc-1, anticorps cd324, anticorps cdhe, anticorps ecad, anticorps AA960649, anticorps E-cad, anticorps Ecad, anticorps L-CAM, anticorps Um, anticorps cadherin 1, anticorps si:busm1-180o5.2, anticorps cadherin 1, type 1, E-cadherin (epithelial), anticorps cadherin 1, type 1 S homeolog, anticorps CDH1, anticorps si:busm1-180o5.2, anticorps cdh1, anticorps Cdh1, anticorps cdh1.S
    Sujet
    CDHE, CD324, CDH1, Arc1, ECAD, CDH-E, E-CAD, LCAM, UVO, Cadherin 1 Type 1, Uvomorulin, E-Cadherin, Calcium-Dependent Adhesion Protein, Epithelial, Liver Cell Adhesion Molecule
    Pathways
    Signalisation WNT, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation
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