E-cadherin anticorps

N° du produit ABIN6941295

Cet anticorps anti-E-cadherin Monoclonal Souris (Clone CDH1-1525) (ABIN6941295) détecte spécifiquement E-cadherin dans WB, IHC, IF, StM et ISt. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour E-cadherin anticorps (ABIN6941295)

Antigène: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp E-cadherin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM), Immunostaining (ISt)

Clone: CDH1-1525

Détail du produit anti-E-cadherin anticorps

Specificité: Recognizes a protein of 120-80 kDa, identified as E-cadherin. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as -catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.

Aucune reactivité croisée: Souris, Rat (Rattus)

Purification: Purified by Protein A/G

Immunogène: Recombinant human E-Cadherin protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: LS174T, Raji, HT29, SK-BR3 cells. Prostate or Colon carcinomas.

Known Application: Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur E-cadherin

Antigène: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Autre désignation: CDH1

Poids moléculaire: 120-80kDa (Mature), 135kDa (Precursor)

ID gène: 999

UniProt: P12830

Pathways: Signalisation WNT, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation