E-cadherin anticorps

N° du produit ABIN2854751

Détail du produit anti-E-cadherin anticorps

Antigène: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp E-cadherin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Whole Mount) (IHC (wm))

Réactivité croisée: Chien, Humain, Souris, Rat, Poisson zèbre (Danio rerio)

Attributs du produit: Rabbit Polyclonal antibody to E-Cadherin (cadherin 1, type 1, E-cadherin (epithelial))
E-Cadherin antibody

Purification: Purified by antigen-affinity chromatography.

Classe de qualité: KO Validated

Immunogène: Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: MCF-7

Validation: Comparison, KO/KD, Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.29 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-E-cadherin (ABIN2854751)

Bray, Elgamal, Jiang, Wright, Sutaria, Badawi, Borcyk, Liu, Fredenburg, Campbell-Thompson, Schmittgen: "Loss of RE-1 silencing transcription factor accelerates exocrine damage from pancreatic injury." dans: Cell death & disease, Vol. 11, Issue 2, pp. 138, (2020) (PubMed).

Zhang, Fang, Chen, Zhao, Wu, Pan: "Qingshen Buyang Formula Attenuates Renal Fibrosis in 5/6 Nephrectomized Rats via Inhibiting EMT and Wnt/β-Catenin Pathway." dans: Evidence-based complementary and alternative medicine : eCAM, Vol. 2019, pp. 5370847, (2019) (PubMed).

Chu, Lee, Lee, Wang, Huang, Lan, Lin, Lin, Tian, Liu, Chow: "MiR-338-5p promotes metastasis of colorectal cancer by inhibition of phosphatidylinositol 3-kinase, catalytic subunit type 3-mediated autophagy pathway." dans: EBioMedicine, Vol. 43, pp. 270-281, (2019) (PubMed).

Ahsan, Singh, Rocha, Huang, Prince: "Prickle1 is required for EMT and migration of zebrafish cranial neural crest." dans: Developmental biology, Vol. 448, Issue 1, pp. 16-35, (2019) (PubMed).

Bacci, Aiello, Ripoli, Loria, Pugliese, Pierconti, Rotili, Strigari, Pinto, Bassi, Mai, Grassi, Pontecorvi, Falcioni, Farsetti, Nanni: "H19-Dependent Transcriptional Regulation of β3 and β4 Integrins Upon Estrogen and Hypoxia Favors Metastatic Potential in Prostate Cancer." dans: International journal of molecular sciences, Vol. 20, Issue 16, (2019) (PubMed).

Wei, Liu, Ren, Liu, Cai, Zhu, Jin, He, Yu: "Effects of bradykinin on TGF‑β1‑induced epithelial‑mesenchymal transition in ARPE‑19 cells." dans: Molecular medicine reports, Vol. 17, Issue 4, pp. 5878-5886, (2018) (PubMed).

Yang, Fu, Yao, Hou, Xue: "Runx3 is a key modulator during the epithelial-mesenchymal transition of alveolar type II cells in animal models of BPD." dans: International journal of molecular medicine, Vol. 40, Issue 5, pp. 1466-1476, (2018) (PubMed).

Chamard-Jovenin, Thiebaut, Chesnel, Bresso, Morel, Smail-Tabbone, Devignes, Boukhobza, Dumond: "Low-Dose Alkylphenol Exposure Promotes Mammary Epithelium Alterations and Transgenerational Developmental Defects, But Does Not Enhance Tumorigenic Behavior of Breast Cancer Cells." dans: Frontiers in endocrinology, Vol. 8, pp. 272, (2017) (PubMed).

Thiebaut, Chamard-Jovenin, Chesnel, Morel, Djermoune, Boukhobza, Dumond: "Mammary epithelial cell phenotype disruption in vitro and in vivo through ERalpha36 overexpression." dans: PLoS ONE, Vol. 12, Issue 3, pp. e0173931, (2017) (PubMed).

Lee, Chen, Chen, Yang, Huang, Liu: "Reverse epithelial-mesenchymal transition contributes to the regain of drug sensitivity in tyrosine kinase inhibitor-resistant non-small cell lung cancer cells." dans: PLoS ONE, Vol. 12, Issue 7, pp. e0180383, (2017) (PubMed).

Détails sur E-cadherin

Antigène: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Autre désignation: cadherin 1 (CDH1 Produits)

Synonymes: anticorps Arc-1, anticorps CD324, anticorps CDHE, anticorps ECAD, anticorps LCAM, anticorps UVO, anticorps E-cadherin, anticorps cdh1, anticorps si:dz180o5.2, anticorps uvo, anticorps lcam, anticorps E-Cad, anticorps XBcad, anticorps l-cam, anticorps xcdh1, anticorps cdhc-A, anticorps xb-cad, anticorps XTCAD-1, anticorps c-cadherin, anticorps cadherin-1, anticorps uvomorulin, anticorps XB-cadherin, anticorps arc-1, anticorps cd324, anticorps cdhe, anticorps ecad, anticorps AA960649, anticorps E-cad, anticorps Ecad, anticorps L-CAM, anticorps Um, anticorps cadherin 1, anticorps si:busm1-180o5.2, anticorps cadherin 1, type 1, E-cadherin (epithelial), anticorps cadherin 1, type 1 S homeolog, anticorps CDH1, anticorps si:busm1-180o5.2, anticorps cdh1, anticorps Cdh1, anticorps cdh1.S

Sujet: This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites.

Cellular Localization: Cell junction , Cell membrane

Poids moléculaire: 97 kDa

ID gène: 999

UniProt: P12830

Pathways: Signalisation WNT, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation