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RAF1 anticorps (AA 342-619)

Cet anticorps Lapin Polyclonal détecte spécifiquement RAF1 dans WB, IHC, ICC et IP. Il présente une réactivité envers Humain.
N° du produit ABIN1858489

Aperçu rapide pour RAF1 anticorps (AA 342-619) (ABIN1858489)

Antigène

Voir toutes RAF1 Anticorps
RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Reactivité

  • 426
  • 200
  • 130
  • 14
  • 12
  • 12
  • 9
  • 9
  • 5
  • 4
  • 4
  • 2
  • 2
  • 2
  • 2
  • 1
Humain

Hôte

  • 459
  • 15
  • 2
  • 2
Lapin

Clonalité

  • 425
  • 53
Polyclonal

Conjugué

  • 190
  • 34
  • 27
  • 26
  • 16
  • 15
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
  • 10
Cet anticorp RAF1 est non-conjugé

Application

  • 283
  • 195
  • 104
  • 104
  • 90
  • 73
  • 38
  • 28
  • 28
  • 24
  • 16
  • 12
  • 12
  • 3
  • 2
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
  • Épitope

    • 49
    • 46
    • 34
    • 32
    • 27
    • 24
    • 24
    • 23
    • 21
    • 19
    • 18
    • 15
    • 14
    • 13
    • 12
    • 8
    • 8
    • 8
    • 7
    • 6
    • 6
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 342-619

    Fonction

    Polyclonal Antibody to C-Raf Proto Oncogene Serine/Threonine Protein Kinase (Raf-1)

    Specificité

    The antibody is a rabbit polyclonal antibody raised against Raf-1. It has been selected for its ability to recognize Raf-1 in immunohistochemical staining and western blotting.

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogène

    CRAF (Trp342-Ser619)

    Isotype

    IgG
  • Indications d'application

    Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

    Commentaires

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    500 μg/mL

    Buffer

    PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

    Agent conservateur

    Dithiothreitol (DTT), ProClin

    Précaution d'utilisation

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Conseil sur la manipulation

    Avoid repeated freeze-thaw cycles.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Date de péremption

    12 months
  • Antigène

    RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

    Autre désignation

    Raf-1

    Sujet

    RAF1, CRAF, V-raf-1 Murine Leukemia Viral Oncogene Homolog 1, Proto-oncogene c-RAF

    Pathways

    Signalisation MAPK, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling
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