This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogène
This POLR2G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-154 amino acids from the C-terminal region of human POLR2G.
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Date de péremption
6 months
Cojocaru, Jeronimo, Forget, Bouchard, Bergeron, Côte, Poirier, Greenblatt, Coulombe: "Genomic location of the human RNA polymerase II general machinery: evidence for a role of TFIIF and Rpb7 at both early and late stages of transcription." dans: The Biochemical journal, Vol. 409, Issue 1, pp. 139-47, (2007) (PubMed).
Ujvári, Luse: "RNA emerging from the active site of RNA polymerase II interacts with the Rpb7 subunit." dans: Nature structural & molecular biology, Vol. 13, Issue 1, pp. 49-54, (2006) (PubMed).
Meka, Werner, Cordell, Onesti, Brick: "Crystal structure and RNA binding of the Rpb4/Rpb7 subunits of human RNA polymerase II." dans: Nucleic acids research, Vol. 33, Issue 19, pp. 6435-44, (2005) (PubMed).
Zhou, Deng, Kashanchi, Brady, Shatkin, Kumar: "The Tat/TAR-dependent phosphorylation of RNA polymerase II C-terminal domain stimulates cotranscriptional capping of HIV-1 mRNA." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 22, pp. 12666-71, (2003) (PubMed).
Antigène
POLR2G
(Polymerase (RNA) II (DNA Directed) Polypeptide G (POLR2G))
This gene encodes the seventh largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The protein functions in transcription initiation, and is also thought to help stabilize transcribing polyermase molecules during elongation.