Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogène
This PIAS2 (PIASx1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the C-terminal region of human PIAS2 (PIASx1).
PIAS2
Reactivité: Humain, Souris, Rat, Singe
WB, ELISA
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
WB: 1:1000. IHC-P: 1:50~100
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Date de péremption
6 months
Jones, Fusi, Higham, Abdel-Hafiz, Horwitz, Lam, Brosens: "Regulation of the SUMO pathway sensitizes differentiating human endometrial stromal cells to progesterone." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 44, pp. 16272-7, (2006) (PubMed).
Arora, Liu, He, Kim, Murphy, Murphy, Modlin, Shuai: "PIASx is a transcriptional co-repressor of signal transducer and activator of transcription 4." dans: The Journal of biological chemistry, Vol. 278, Issue 24, pp. 21327-30, (2003) (PubMed).
Wu, Cetinkaya, Munoz-Alonso, von der Lehr, Bahram, Beuger, Eilers, Leon, Larsson: "Myc represses differentiation-induced p21CIP1 expression via Miz-1-dependent interaction with the p21 core promoter." dans: Oncogene, Vol. 22, Issue 3, pp. 351-60, (2003) (PubMed).
Liu, Liao, Rao, Kushner, Chung, Chang, Shuai: "Inhibition of Stat1-mediated gene activation by PIAS1." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 18, pp. 10626-31, (1998) (PubMed).
Antigène
PIAS2
(Protein Inhibitor of Activated STAT, 2 (PIAS2))
anticorps MGC83751, anticorps piasx, anticorps wu:fi05f09, anticorps PIAS2, anticorps 6330408K17Rik, anticorps AI462206, anticorps ARIP3, anticorps AU018068, anticorps Dib, anticorps Miz1, anticorps PIASxalpha, anticorps PIASxb, anticorps PIASxbeta, anticorps SIZ2, anticorps DIP, anticorps MIZ, anticorps MIZ1, anticorps PIASX, anticorps PIASX-ALPHA, anticorps PIASX-BETA, anticorps ZMIZ4, anticorps protein inhibitor of activated STAT 2 L homeolog, anticorps protein inhibitor of activated STAT 2, anticorps protein inhibitor of activated STAT, 2, anticorps pias2.L, anticorps PIAS2, anticorps pias2, anticorps Pias2
Sujet
PIASX functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. PIASX binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. This antibody is directed against an epitope specific to PIAS2-alpha. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha.