AKT2 anticorps (pSer474)
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- Antigène Voir toutes AKT2 Anticorps
- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
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Épitope
- pSer474
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Reactivité
- Humain, Souris
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp AKT2 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificité
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. Akt2 (phospho-Ser474) antibody detects endogenous levels of Akt2 only when phosphorylated at serine 474.
- Purification
- Immunoaffinity chromatography.
- Immunogène
- The antiserum was produced against synthesized phosphopeptide derived from human Akt2 around the phosphorylation site of serine 474 (Q-F-SP-Y-S).
- Top Product
- Discover our top product AKT2 Anticorps primaire
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- Indications d'application
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Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- -20 °C
- Stockage commentaire
- Store the antibody (in aliquots) at -20 °C.
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- Antigène
- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
- Autre désignation
- AKT2 / PKB beta (AKT2 Produits)
- Synonymes
- anticorps AKT2, anticorps pkbb, anticorps pkbbeta, anticorps pkbss, anticorps prkbb, anticorps rac-beta, anticorps v-akt2, anticorps 2410016A19Rik, anticorps AW554154, anticorps PKB, anticorps PKBbeta, anticorps HIHGHH, anticorps PKBB, anticorps PKBBETA, anticorps PRKBB, anticorps RAC-BETA, anticorps cb945, anticorps akt2-a, anticorps AKT serine/threonine kinase 2, anticorps v-akt murine thymoma viral oncogene homolog 2, anticorps thymoma viral proto-oncogene 2, anticorps v-akt murine thymoma viral oncogene homolog 2 L homeolog, anticorps AKT2, anticorps akt2, anticorps Akt2, anticorps akt2.L
- Sujet
- The serine/threonine kinase Akt (protein kinase B or PKB) has a central role in the regulation of several signaling pathways controlling cell proliferation, apoptosis, angiogenesis, and diabetes. In humans, there are three genes in the "Akt family": Akt1, Akt2, and Akt3. AKT2 is a putative oncogene and is a general protein kinase capable of phophorylating several known proteins. AKT2 is amplified and overexpressed in some human carcinomas. AKT2 acts primarily as a regulator of glucose metabolism.Synonyms: Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, RAC-beta serine/threonine-protein kinase
- ID gène
- 208
- NCBI Accession
- NP_001617
- UniProt
- P31751
- Pathways
- Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Signalisation TLR, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling
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