LCK anticorps (pTyr394)

N° du produit ABIN196860

Détail du produit anti-LCK anticorps

Antigène: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Épitope: pTyr394

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LCK est non-conjugé

Application: Western Blotting (WB)

Specificité: This antibody detects endogenous levels of Lck only when phosphorylated at Tyrosine 394.

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse.

Purification: Immunoaffinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around the phosphorylation site of Tyrosine 394 (N-E-pY-T-A) derived from Human Lck.

Information d'application

Indications d'application: Suitable for use in Western blot (1: 500approx. 1: 1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at -20 °C.

Détails sur LCK

Antigène: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Autre désignation: LCK (LCK Produits)

Synonymes: anticorps zgc:136695, anticorps LCK, anticorps Hck-3, anticorps Lsk, anticorps Lskt, anticorps p56, anticorps p56Lck, anticorps LSK, anticorps YT16, anticorps p56lck, anticorps pp58lck, anticorps P56LCK, anticorps tkl, anticorps Lck1, anticorps Lcktkr, anticorps LCK proto-oncogene, Src family tyrosine kinase, anticorps lymphocyte protein tyrosine kinase, anticorps lck, anticorps LCK, anticorps Lck

Sujet: Lck is a lymphoid specific cytosolic protein tyrosine kinase (PTK), which is essential for T cell development and function. It is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T cell antigen receptor (TCR) linked signal transduction pathways. Association of the TCR with a peptide antigen bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated Lck to the vicinity of the TCR/CD3 complex. Lck then phosphorylates tyrosine residues within the immunoreceptor tyrosine based activation motifs (ITAMs) in the cytoplasmic tails of the TCR chains and CD3 subunits. The phosphoITAMs serve as docking sites for Src homology domain 2 (SH2) containing molecules, predominantly ZAP 70 and Syk. Only then can ZAP 70 undergo tyrosine phosphorylation, become enzymatically active and further phosphorylate downstream effector molecules. In addition, Lck contributes to signaling by other receptor molecules. Lck is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature TCR.Synonyms: LSK, Lymphocyte cell-specific protein-tyrosine kinase, Proto-oncogene tyrosine-protein kinase LCK, T cell-specific protein-tyrosine kinase, p56-LCK

Poids moléculaire: 56 kDa

ID gène: 3932

NCBI Accession: NP_001036236

UniProt: P06239

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling