HSP27 anticorps (Ser15)

N° du produit ABIN197345

Détail du produit anti-HSP27 anticorps

Antigène: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Épitope: Ser15

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HSP27 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. HSP27 antibody AP02671PU detects endogenous levels of total HSP27 protein.

Purification: Immunoaffinity chromatography.

Immunogène: The antiserum was produced against synthesized non-phosphopeptide derived from human HSP27 around the phosphorylation site of serine 15 (G-P-Sp-W-D).

Information d'application

Indications d'application: Western blot (1: 500-1: 1000)Immunohistochemistry (1: 50-1: 100)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at -20 °C.

Détails sur HSP27

Antigène: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Autre désignation: HSPB1 / HSP27 (HSPB1 Produits)

Synonymes: anticorps CMT2F, anticorps HMN2B, anticorps HS.76067, anticorps HSP27, anticorps HSP28, anticorps Hsp25, anticorps SRP27, anticorps 27kDa, anticorps Hsp27, anticorps cb153, anticorps cb660, anticorps hsp1, anticorps hsp25, anticorps hsp27, anticorps id:ibd2821, anticorps sb:cb660, anticorps zgc:103437, anticorps HSPB1, anticorps cmt2f, anticorps hs.76067, anticorps hsp28, anticorps heat shock protein family B (small) member 1, anticorps heat shock protein 1, anticorps heat shock protein, alpha-crystallin-related, 1, anticorps heat shock protein family B (small) member 1 L homeolog, anticorps HSPB1, anticorps Hspb1, anticorps hspb1, anticorps hspb1.L

Sujet: Hsp27, also referred to as the Estrogen regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. Hsp27 synthesis is induced by elevated temperature, as well as estrogen in hormone responsive cells. This protein is involved in stress resistance and actin organization. Interestingly, human HSP27 also shares greater than 50 % homology with low molecular weight Drosophila HSP's and mammalian a-crystalline lens protein. Because of the estrogen responsive nature of Hsp27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. This work has led to the suggestion that Hsp27 may be a useful marker in classifying various hormone sensitive tumors.Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP25, HSP28, Heat shock 27 kDa protein, Heat shock protein beta-1, SRP27, Stress-responsive protein 27

ID gène: 3315

NCBI Accession: NP_001531

UniProt: P04792

Pathways: Signalisation MAPK, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling