DOK2 anticorps (AA 297-301)

N° du produit ABIN197540

Détail du produit anti-DOK2 anticorps

Antigène: DOK2

Épitope: AA 297-301

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DOK2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: This antibody detects endogenous levels of total p56Dok-2 protein.

Purification: Affinity chromatography

Immunogène: The antiserum was produced against synthesized non-phosphopeptide derived from Human p56Dok-2 (aa 297~301) around the phosphorylation site of Tyrosine 299 (G-E-Yp-A-V).

Information d'application

Indications d'application: Western blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at -20 °C.

Détails sur DOK2

Antigène: DOK2

Autre désignation: DOK2 / p56 Dok2 (DOK2 Produits)

Synonymes: anticorps DokR, anticorps Frip, anticorps Dok-2, anticorps p56DOK, anticorps p56dok-2, anticorps docking protein 2, anticorps DOK2, anticorps Dok2

Sujet: Docking proteins interact with receptor tyrosine kinases and mediate particular biological responses using signal transduction. DOK2 acts as a multiple docking protein downstream of receptor or non-receptor tyrosine kinases. By this mechanism it acts to negatively regulate signal transduction and cell proliferation controlled by cytokines in a feedback loop. DOK2 is highly expressed in cells and tissues of hematopoietic origin as well as in lung. Expression of bcr/abl induces additional tyrosine phosphorylation of the DOK1 and DOK2 proteins and their association with Ras-GAP. Thus, it is suspected that DOK association regulates GAP activity toward Ras and that the DOK proteins serve as mediators of bcr-abl signaling. The role of DOK proteins in bcr-abl regulation may also be implicated in chronic myelogenous leukemia (CML), which is characterized by a Philadelphia chromosome translocation t(9,22). Such a mutation would result in a p210-bcr/abl chimeric protein-tyrosine kinase which has been found in many CML cases.Synonyms: Docking protein 2, Downstream of tyrosine kinase 2, p56(dok-2), p56Dok-2

ID gène: 9046

NCBI Accession: NP_003965

UniProt: O60496