TNF alpha anticorps

N° du produit ABIN199734

Cet anticorps anti-TNF alpha Monoclonal Souris (ABIN199734) détecte spécifiquement TNF alpha dans WB, ELISA, ICC et Neut. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour TNF alpha anticorps (ABIN199734)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TNF alpha est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Neutralization (Neut)

Détail du produit anti-TNF alpha anticorps

Specificité: Recognizes recombinant human TNF-a. Cross-reactivity: rhTNF-b, rmTNF-a, rrTNF-a, rpTNF-a, rhTNF RI, rhTNF RII, rmTNF RI and rmTNF RII: >0.05 % .

Purification: Protein G purified

Immunogène: RhTNR-a expressed in E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Information d'application

Indications d'application: Approved: ELISA (4 μg/mL), ICC, Neut, WB (1 - 2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Neutralization. ELISA: 4 μg/mL as a capture antibody. Use 100 μL/well. Western Blot: ~1-2 μg/mL. The detection limit for rhTNFa is 5 ng/lane under non-reducing and reducing conditions using colorimetric detection. 5-50-fold increased sensitivity can be obtained using ECL. Neutralization of Bioactivity: 0.04-0.08 μg/mL typically neutralizes 50 % of the bioactivity of 0.25 ng/mL of rhTNF-a.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 5 % trehalose.

Conseil sur la manipulation: avoid freeze thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Détails sur TNF alpha

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF Alpha

Sujet: Name/Gene ID: TNF
Family: TNF

Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2

ID gène: 7124

UniProt: P01375

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome