TNF alpha anticorps
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- Antigène Voir toutes TNF alpha Anticorps
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp TNF alpha est non-conjugé
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Application
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Neutralization (Neut)
- Specificité
- Recognizes recombinant human TNF-a. Cross-reactivity: rhTNF-b, rmTNF-a, rrTNF-a, rpTNF-a, rhTNF RI, rhTNF RII, rmTNF RI and rmTNF RII: >0.05 % .
- Purification
- Protein G purified
- Immunogène
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RhTNR-a expressed in E. coli.
Type of Immunogen: Recombinant protein - Isotype
- IgG1
- Top Product
- Discover our top product TNF alpha Anticorps primaire
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- Indications d'application
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Approved: ELISA (4 μg/mL), ICC, Neut, WB (1 - 2 μg/mL)
Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Neutralization. ELISA: 4 μg/mL as a capture antibody. Use 100 μL/well. Western Blot: ~1-2 μg/mL. The detection limit for rhTNFa is 5 ng/lane under non-reducing and reducing conditions using colorimetric detection. 5-50-fold increased sensitivity can be obtained using ECL. Neutralization of Bioactivity: 0.04-0.08 μg/mL typically neutralizes 50 % of the bioactivity of 0.25 ng/mL of rhTNF-a. - Commentaires
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.2, 5 % trehalose.
- Conseil sur la manipulation
- avoid freeze thaw cycles.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- Antigène
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- Autre désignation
- TNF Alpha (TNF alpha Produits)
- Synonymes
- anticorps DIF, anticorps TNF-alpha, anticorps TNFA, anticorps TNFSF2, anticorps RATTNF, anticorps Tnfa, anticorps tnf, anticorps TNF-a, anticorps TNFalpha, anticorps Tnfsf1a, anticorps TNFa, anticorps cTNF, anticorps Tnf-alpha, anticorps tnfa-like, anticorps TNF-ALPHA, anticorps dif, anticorps tnfa, anticorps xtnf, anticorps tnfsf2, anticorps tnf-alpha, anticorps Cachectin, anticorps tumor necrosis factor, anticorps tumor necrosis factor b (TNF superfamily, member 2), anticorps tumor necrosis factor alpha, anticorps tumor necrosis factor a (TNF superfamily, member 2), anticorps TNF, anticorps Tnf, anticorps tnf, anticorps tnfb, anticorps tnf-alpha, anticorps LOC103694380, anticorps tnfa
- Sujet
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Name/Gene ID: TNF
Family: TNF
Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2 - ID gène
- 7124
- UniProt
- P01375
- Pathways
- Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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