BRAF anticorps (AA 754-765)

N° du produit ABIN205677

Cet anticorps anti-BRAF est un anticorps Lapin Polyclonal détectant BRAF dans WB. Adapté pour Humain, Singe, Chien et Cheval.

Aperçu rapide pour BRAF anticorps (AA 754-765) (ABIN205677)

Antigène: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Reactivité: Humain, Singe, Chien, Cheval

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BRAF est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-BRAF anticorps

Épitope: AA 754-765

Specificité: Recognizes full length recombinant human BRaf (plus 6His tag, Mr ~94kD) and B-Raf splice variants in cell lysates (Mr ~68-94kD). Species cross-reactivity: Rat and mouse.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Elephant, Panda, Dog, Horse, Opossum (100%) Mouse, Porcine, Turkey, Rabbit, Chicken, Platypus (92%) Bat (83%).

Purification: Protein A purified

Immunogène: Synthetic peptide corresponding to amino acids 754-765 (C-IQAGGYGAFPVH) of human B-Raf, with an N-terminal cysteine added for conjugation to KLH. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Elephant, Panda, Dog, Horse, Opossum (100%), Mouse, Porcine, Turkey, Rabbit, Chicken, Platypus (92%), Bat (83%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Information d'application

Indications d'application: Approved: WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot, MAP Kinase Activation and MBP Phosphorylation assays. Western Blot: 1-2 μg/mL detects B-Raf splice variants in RIPA lysates from PC12 cells and the full length purified B-Raf, active enzyme. MAP Kinase Activation and MBP Phosphorylation: 4 μg.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide 30 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur BRAF

Antigène: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Autre désignation: BRAF / B-Raf

Sujet: Name/Gene ID: BRAF
Subfamily: Raf
Family: Protein Kinase

Synonyms: BRAF, 94 kDa B-raf protein, B-raf, BRAF1, B Raf, Proto-oncogene B-Raf, p94, B-RAF1, NS7, ONCOGENE BRAF, RAFB1

ID gène: 673

UniProt: P15056

Pathways: Signalisation MAPK, Signalisation RTK, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy