BRAF anticorps (AA 754-765)
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- Antigène Voir toutes BRAF Anticorps
- BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))
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Épitope
- AA 754-765
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Reactivité
- Humain, Singe, Chien, Cheval
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp BRAF est non-conjugé
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Application
- Western Blotting (WB)
- Specificité
- Recognizes full length recombinant human BRaf (plus 6His tag, Mr ~94kD) and B-Raf splice variants in cell lysates (Mr ~68-94kD). Species cross-reactivity: Rat and mouse.
- Homologie
- Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Elephant, Panda, Dog, Horse, Opossum (100%) Mouse, Porcine, Turkey, Rabbit, Chicken, Platypus (92%) Bat (83%).
- Purification
- Protein A purified
- Immunogène
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Synthetic peptide corresponding to amino acids 754-765 (C-IQAGGYGAFPVH) of human B-Raf, with an N-terminal cysteine added for conjugation to KLH. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Elephant, Panda, Dog, Horse, Opossum (100%), Mouse, Porcine, Turkey, Rabbit, Chicken, Platypus (92%), Bat (83%).
Type of Immunogen: Synthetic peptide - KLH conjugated - Isotype
- IgG
- Top Product
- Discover our top product BRAF Anticorps primaire
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- Indications d'application
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Approved: WB (1 - 2 μg/mL)
Usage: Suitable for use in Western Blot, MAP Kinase Activation and MBP Phosphorylation assays. Western Blot: 1-2 μg/mL detects B-Raf splice variants in RIPA lysates from PC12 cells and the full length purified B-Raf, active enzyme. MAP Kinase Activation and MBP Phosphorylation: 4 μg. - Commentaires
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide 30 % glycerol.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeat freeze-thaw cycles.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Antigène
- BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))
- Autre désignation
- BRAF / B-Raf (BRAF Produits)
- Synonymes
- anticorps B-RAF1, anticorps BRAF1, anticorps NS7, anticorps RAFB1, anticorps C-RMIL, anticorps RMIL, anticorps B-Raf, anticorps braf1, anticorps rafb1, anticorps b-raf1, anticorps sierrabraf, anticorps BRAF, anticorps 9930012E13Rik, anticorps AA120551, anticorps AA387315, anticorps AA473386, anticorps B-raf, anticorps Braf-2, anticorps Braf2, anticorps C230098H17, anticorps C87398, anticorps D6Ertd631e, anticorps braf, anticorps fc48g01, anticorps wu:fc48g01, anticorps B-Raf proto-oncogene, serine/threonine kinase, anticorps B-Raf proto-oncogene, serine/threonine kinase S homeolog, anticorps Braf transforming gene, anticorps si:dkey-222f8.3, anticorps BRAF, anticorps Braf, anticorps braf.S, anticorps braf, anticorps si:dkey-222f8.3
- Sujet
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Name/Gene ID: BRAF
Subfamily: Raf
Family: Protein Kinase
Synonyms: BRAF, 94 kDa B-raf protein, B-raf, BRAF1, B Raf, Proto-oncogene B-Raf, p94, B-RAF1, NS7, ONCOGENE BRAF, RAFB1 - ID gène
- 673
- UniProt
- P15056
- Pathways
- Signalisation MAPK, Signalisation RTK, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy
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