EGF anticorps (Extracellular Domain)

N° du produit ABIN205919

L’anticorps Lapin Polyclonal anti-EGF a été validé pour WB, IHC, ELISA, IHC (p) et Func. Il convient pour détecter EGF dans des échantillons de Humain.

Aperçu rapide pour EGF anticorps (Extracellular Domain) (ABIN205919)

Antigène: EGF (Epidermal Growth Factor (EGF))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EGF est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)

Détail du produit anti-EGF anticorps

Épitope: Extracellular Domain

Specificité: Recognizes human Epidermal Growth Factor, a potent stimulator and regulator of the proliferation of epidermal and epithelial cells, expressed by a variety of cells. The effects of EGF are initiated through tyrosine kinase activity, following the binding of EGF to the extracellular domain of the ~170 kD EGF receptor (EGFR), which also binds other EGF-like ligands with high affinity, including TGF-a (transforming growth factor alpha), VGF (vaccinia virus growth factor) and Betacellulin, expressed in the pancreas, small intestine and by certain tumor cells. Receptor activation through EGF binding triggers several signal transduction pathways, including the JAK/STAT and P13K/AKT pathways.

Purification: Purified

Immunogène: Recombinant human EGF.

Type of Immunogen: Recombinant protein

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA (0.5 - 2 μg/mL), Func (0.25 - 0.35 μg/mL), IHC, IHC-P (0.25 - 1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This product requires protein digestion pre-treatment of paraffin sections e. g. trypsin or pronase. Histology Positive Control Tissue: Human skin ELISA: LS-C42950 may be used in an indirect ELISA system or as a capture antibody in a sandwich ELISA for human EGF in combination with LS-C42951 as a detection reagent.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled Water. For long term storage the addition of 0.09 % sodium azide is recommended. For functional studies do not add azide.

Concentration: Lot specific

Buffer: Lyophilized, PBS,  pH 7.4.

Détails sur EGF

Antigène: EGF (Epidermal Growth Factor (EGF))

Autre désignation: EGF

Sujet: Name/Gene ID: EGF

Synonyms: EGF, Beta-urogastrone, HOMG4, URG, Epidermal growth factor, Pro-epidermal growth factor

ID gène: 1950

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation