STAT5B anticorps (AA 774-787)

N° du produit ABIN210645

Cet anticorps anti-STAT5B est un anticorps Lapin Polyclonal détectant STAT5B dans WB et IP. Adapté pour Humain, Souris, Rat, Boeuf (Vache), Chien, Cheval, Porc et Hamster.

Aperçu rapide pour STAT5B anticorps (AA 774-787) (ABIN210645)

Antigène: STAT5B (Signal Transducer and Activator of Transcription 5B (STAT5B))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Cheval, Porc, Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp STAT5B est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Détail du produit anti-STAT5B anticorps

Épitope: AA 774-787

Specificité: Recognizes the 90-92kD forms of STAT5B protein. Species cross-reactivity: Human and mouse.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Horse, Pig (100%) Panda, Bat, Chicken, Xenopus (93%) Opossum (86%).

Purification: Protein A purified

Immunogène: Amino acids 774-787 of human STAT5B (GRPMDSQWIPHAQS). The peptide was coupled to KLH prior to immunization. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Horse, Pig (100%), Panda, Bat, Chicken, Xenopus (93%), Opossum (86%).

Isotype: IgG

Information d'application

Indications d'application: Approved: IP, WB

Usage: Western Blot: 0.5-2 mg/mL detects STAT5B (doublet of 90kD and 92kD) in. human A431 and mouse 3T3 fibroblasts using enhanced chemiluminescence with a five minute exposure. Immunoprecipitation: 4 μg immunoprecipitates STAT5B from A431 carcinoma cells and a. previous lot immunoprecipitated STAT5B from human Raji cells. A RIPA lysate of human A431 cells (500 μg protein) was incubated with 4 μg of anti-STAT 5B Immune complexes were precipitated with protein A agarose and analyzed by immunoblotting with anti- STAT 5B. Bands were visualized using a goat anti-rabbit IgG secondary antibody conjugated to HRP in conjunction with enhanced chemiluminescence detection. Positive Antigen Control: mouse 3T3 cell lysate. Add 2.5 μL of 2-mercaptoethanol. per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Détails sur STAT5B

Antigène: STAT5B (Signal Transducer and Activator of Transcription 5B (STAT5B))

Autre désignation: STAT5B / STAT

Sujet: Name/Gene ID: STAT5B

Synonyms: STAT5B, Transcription factor STAT5B, STAT5

ID gène: 6777

UniProt: P51692

Pathways: Signalistation JAK/STAT, Signalisation RTK, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation