CD13 anticorps (Biotin)

N° du produit ABIN2144262

Détail du produit anti-CD13 anticorps (Biotin)

Antigène: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD13 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Attributs du produit: 4AD43 reacts with CD13, 150 kDa aminopeptidase N (APN).

Clone: 4AD43

Isotype: IgG2b

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Procédure de l'essai:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature.
• Repeat step 5.
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur CD13

Antigène: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Autre désignation: CD13 (ANPEP Produits)

Synonymes: anticorps APN, anticorps CD13, anticorps GP150, anticorps LAP1, anticorps P150, anticorps PEPN, anticorps A2AP, anticorps AAP, anticorps ALPHA-2-PI, anticorps API, anticorps PLI, anticorps AAT1, anticorps ALT1, anticorps GPT1, anticorps Gpt1, anticorps Apn, anticorps pepN, anticorps SC8E4A.13, anticorps 1300007J06Rik, anticorps 2310022B03Rik, anticorps ALT, anticorps Gpt-1, anticorps AP-M, anticorps AP-N, anticorps Cd13, anticorps Apm, anticorps KZP, anticorps Lap1, anticorps rAPN, anticorps APDE, anticorps p150, anticorps anpep, anticorps fb64c05, anticorps wu:fb64c05, anticorps zgc:136771, anticorps alanyl aminopeptidase, membrane, anticorps serpin family F member 2, anticorps glutamic--pyruvic transaminase, anticorps aminopeptidase N, anticorps aminopeptidase, anticorps aminopeptidase n, anticorps glutamic pyruvic transaminase, soluble, anticorps alanyl (membrane) aminopeptidase, anticorps membrane alanyl aminopeptidase, anticorps alanyl (membrane) aminopeptidase b, anticorps alanyl aminopeptidase, membrane L homeolog, anticorps ANPEP, anticorps SERPINF2, anticorps GPT, anticorps Gpt, anticorps Apn2, anticorps Apn1, anticorps SCO2643, anticorps pepN, anticorps Shewana3_0897, anticorps Alvin_1024, anticorps Ndas_3413, anticorps Saut_1544, anticorps Fbal_1877, anticorps ML5_3424, anticorps Despr_1988, anticorps Mesop_5365, anticorps Anpep, anticorps LOC101744250, anticorps anpepb, anticorps anpep.L

Sujet: CD13 is expressed on the surface of early committed progenitors and mature granulocytes and monocytes (GM-CFU), but not on lymphocytes, platelets or erythrocytes. It is also expressed on endothelial cells, epithelial cells, bone marrow stroma cells, and osteoclasts, as well as a small proportion of LGL lymphocytes. CD13 acts as a receptor for specific strains of RNA viruses and plays an important function in the interaction between human cytomegalovirus (CMV) and its target cells.

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones