CD20 anticorps (PE-Cy5)
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- Antigène Voir toutes CD20 (MS4A1) Anticorps
- CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD20 est conjugé à/à la PE-Cy5
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Application
- Flow Cytometry (FACS)
- Attributs du produit
- 4ABCA/B20 reacts with human CD20 (B1), 37/35 kDa protien, which is expressed on pre-B cells and mature B cells but not on plasma cells.
- Clone
- 4ABCA-b20
- Isotype
- IgG2a
- Top Product
- Discover our top product MS4A1 Anticorps primaire
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Procédure de l'essai
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
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- Antigène
- CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
- Autre désignation
- CD20 (MS4A1 Produits)
- Synonymes
- anticorps B1, anticorps Bp35, anticorps CD20, anticorps CVID5, anticorps LEU-16, anticorps MS4A2, anticorps S7, anticorps AA960661, anticorps Cd20, anticorps Ly-44, anticorps Ms4a2, anticorps MS4A1, anticorps bp35, anticorps cd20, anticorps ms4a2, anticorps leu-16, anticorps ms4a4c, anticorps cd20-like, anticorps membrane spanning 4-domains A1, anticorps membrane-spanning 4-domains, subfamily A, member 1, anticorps B-lymphocyte antigen CD20, anticorps MS4A1, anticorps Ms4a1, anticorps ms4a1, anticorps LOC101694281
- Sujet
- The CD20 antigen can also be detected at low levels on a subset of peripheral blood T-cells. CD20 regulates B-cell activation and proliferation by regulating transmembrane Ca++ conductance and cell-cycle progression.This clone is cross reactive with non-human primate .* CD20 antibody has been used for lymphoma diagnosis and therapy.
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