CD15 anticorps (FITC)
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- Antigène Voir toutes CD15 (FUT4) Anticorps
- CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD15 est conjugé à/à la FITC
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Application
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Attributs du produit
- The 4AHI98 monoclonal antibody reacts with human CD15, Lewis X. This 3-fucosyl-N-acetyllactosamine carbohydrate moiety is expressed by granulocytes. Monocytes express this structure at varying degree while lymphocytes are negative.
- Clone
- 4AHI98
- Isotype
- IgM
- Top Product
- Discover our top product FUT4 Anticorps primaire
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Procédure de l'essai
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
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- Antigène
- CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))
- Autre désignation
- CD15 (FUT4 Produits)
- Synonymes
- anticorps AI451562, anticorps CD15, anticorps FAL, anticorps FucT-IV, anticorps LeX, anticorps SSEA-1, anticorps Ssea1, anticorps ELFT, anticorps FCT3A, anticorps FUC-TIV, anticorps FUTIV, anticorps Fuct, anticorps fucosyltransferase 4, anticorps Fut4, anticorps FUT4
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