CD81 anticorps (FITC)

N° du produit ABIN2144695

Détail du produit anti-CD81 anticorps (FITC)

Antigène: CD81

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD81 est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Attributs du produit: 4A1.3.3.22 reacts with the CD81, a target for anti-proliferative antigen (TAPA-1) with 26 kDa MW, which ia a member of the TM4SF tetraspanin family. CD81 is broadly expressed on hemapoietic cells and enothelial and epithelial cells, but absent from erythrocytes and platelets as well as neutrophils. CD81 play role as a member of CD19/CD21/Leu-13 signal transdiction complex. It also is reported that anti-TAPA-1 antibody induce protein tyrosine phosphorylation that is prevented by increased intercellular thiol levels.

Clone: 4A1-3-3-22

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Procédure de l'essai:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur CD81

Antigène: CD81

Autre désignation: CD81 (CD81 Produits)

Synonymes: anticorps Tapa-1, anticorps Tapa1, anticorps Tspan28, anticorps CVID6, anticorps S5.7, anticorps TAPA1, anticorps TSPAN28, anticorps CD81 molecule, anticorps CD81 molecule b, anticorps CD81 antigen, anticorps Cd81 molecule, anticorps CD81, anticorps cd81b, anticorps Cd81

Pathways: Inositol Metabolic Process, Hepatitis C