VCAM1 anticorps (FITC)

N° du produit ABIN2144710

Détail du produit anti-VCAM1 anticorps (FITC)

Antigène: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp VCAM1 est conjugé à/à la FITC

Application: Flow Cytometry (FACS)

Attributs du produit: CD106 is a 110 kDa single chain type I glycoprotein also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-110. It is expressed at high levels on the surface of cytokine-stimulated endothelium and at minimal levels on unstimulated endothelium.VCAM-1 serves as a ligand for the leucocyte integrins alpha 4 beta (CD49d/CD29 complex).A function of CD106 is to provide a costimulation signal in T cell activation. Reactivity with other species not tested.

Clone: 4AP8B1

Isotype: IgG2a

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Procédure de l'essai:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur VCAM1

Antigène: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Autre désignation: CD106 (VCAM1 Produits)

Synonymes: anticorps CD106, anticorps INCAM-100, anticorps Vcam-1, anticorps VCAM1B, anticorps fi76d06, anticorps wu:fi76d06, anticorps zgc:158875, anticorps VCAM1, anticorps vascular cell adhesion molecule 1, anticorps vascular cell adhesion molecule 1b, anticorps VCAM1, anticorps Vcam1, anticorps vcam1b, anticorps VCAM-1

Pathways: Carbohydrate Homeostasis