HSP27 anticorps

N° du produit ABIN215209

Cet anticorps Souris Monoclonal détecte spécifiquement HSP27 dans WB, IHC, ELISA, IF, IP et ICC. Il présente une réactivité envers Humain et Singe.

Aperçu rapide pour HSP27 anticorps (ABIN215209)

Antigène: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivité: Humain, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HSP27 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Détail du produit anti-HSP27 anticorps

Specificité: Recognizes an ~27kD protein, corresponding to the apparent molecular mass of Hsp27 on SDS-PAGE immunoblots. Species cross-reactivity: Human, monkey and fish (rainbow trout). It does not exhibit reactivity with rodent Hsp25, alphaA, alphaB or beta-crystallin proteins.

Purification: Protein G purified

Immunogène: Purified Hsp24 isolated from MCF-7, a human breast tumor cell line (7). Upon cloning and sequence analysis of the gene encoding the 24kD protein, nucleotide sequence comparison revealed complete identity with the small heat shock protein, Hsp27.

Isotype: IgG1

Information d'application

Indications d'application: Approved: ELISA, ICC (10 μg/mL), IF, IHC (5 μg/mL), IP (12.5 μg/mL), WB

Usage: Suitable for use in ELISA, Western Blot and Immunofluorescence. Western: Blot (Colorimetric): 1 μg/mL. Immunoprecipitation: 12.5 μg/mL. Immunohistochemistry: 5 μg/mL. Immunocytochemistry: 10 μg/mL. Immunoelectron Microscopy: 1:50. Positive control: HeLa Heat Shocked Cell Lysate and Recombinant Human Hsp27 Protein.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 mM PMSF, 50 % glycerol.

Agent conservateur: PMSF

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur HSP27

Antigène: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Autre désignation: HSPB1 / HSP27

Sujet: Name/Gene ID: HSPB1

Synonyms: HSPB1, 28 kDa heat shock protein, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27  kDa protein 1, HSP27, HSP28, HSP 27, Hsp25, SRP27, Stress-responsive protein 27, CMT2F, Heat shock 27 kd protein, Heat shock protein beta-1, HMN2B, HS.76067

ID gène: 3315

UniProt: P04792

Pathways: Signalisation MAPK, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling