HSP27 anticorps (Biotin)

N° du produit ABIN215210

L’anticorps Souris Monoclonal anti-HSP27 a été validé pour WB, IHC, ELISA, IP et ICC. Il convient pour détecter HSP27 dans des échantillons de Humain et Singe.

Aperçu rapide pour HSP27 anticorps (Biotin) (ABIN215210)

Antigène: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivité: Humain, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HSP27 est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Détail du produit anti-HSP27 anticorps (Biotin)

Specificité: Recognizes a ~27kD protein, corresponding to the apparent molecular mass of HSP27 on SDS-PAGE immunoblots. Species cross-reactivity: Human, monkey and fish (rainbow trout). Does not exhibit reactivity with rodent HSP25, alphaA, alphaB or beta-crystallin proteins.

Purification: Protein G purified

Immunogène: Purified HSP24 isolated from MCF-7, a human breast tumor cell line. Upon cloning and sequence analysis of the gene encoding the 24kD protein, nucleotide sequence comparison revealed complete identity with the small heat shock protein, HSP27.

Type of Immunogen: Purified protein

Isotype: IgG1

Information d'application

Indications d'application: Approved: ELISA, ICC (10 μg/mL), IHC (5 μg/mL), IP, WB (1 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunoelectron Microscopy, Apoptosis Induction, and EIA/ELISA. Western Blot (ECL): 1 μg/mL. Immunohistochemistry: 5 μg/mL. immunocytochemistry: 10 μg/mL. Immunoelectron Microscopy: 1:50. Positive control: H1838-01: HeLa Heat Shocked Cell Lysate. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide, 40 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Détails sur HSP27

Antigène: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Autre désignation: HSPB1 / HSP27

Sujet: Name/Gene ID: HSPB1

Synonyms: HSPB1, 28 kDa heat shock protein, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27  kDa protein 1, HSP27, HSP28, HSP 27, Hsp25, SRP27, Stress-responsive protein 27, CMT2F, Heat shock 27 kd protein, Heat shock protein beta-1, HMN2B, HS.76067

ID gène: 3315

UniProt: P04792

Pathways: Signalisation MAPK, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling