TNF alpha anticorps

N° du produit ABIN2191873

Cet anticorps Souris Monoclonal détecte spécifiquement TNF alpha dans WB, FACS, IHC (p) et IHC (fro). Il présente une réactivité envers Humain et a été mentionné dans 4+ publications.

Aperçu rapide pour TNF alpha anticorps (ABIN2191873)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TNF alpha est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 52B83

Détail du produit anti-TNF alpha anticorps

Réactivité croisée (Details): Cross reactivity: Mouse : Yes, Guinea pig : Yes, Rhesus monkey : Yes

Stérilité: 0.2 μm filtered

Isotype: IgG1

Information d'application

Indications d'application: For immunohistochemistry, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. 1

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Références pour anticorps anti-TNF alpha (ABIN2191873)

van der Laan, de Leij, Buurman, Timens, ten Duis: "Tumor necrosis factor alpha (TNFalpha) in human skin: a comparison of different antibodies for immunohistochemistry." dans: Archives of dermatological research, Vol. 293, Issue 5, pp. 226-32, (2001) (PubMed).

Gerspach, Götz, Zimmermann, Kolle, Böttinger, Grell: "Detection of membrane-bound tumor necrosis factor (TNF): an analysis of TNF-specific reagents." dans: Microscopy research and technique, Vol. 50, Issue 3, pp. 243-50, (2000) (PubMed).

Bradding, Mediwake, Feather, Madden, Church, Holgate, Howarth: "TNF alpha is localized to nasal mucosal mast cells and is released in acute allergic rhinitis." dans: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, Vol. 25, Issue 5, pp. 406-15, (1995) (PubMed).

Bradding, Roberts, Britten, Montefort, Djukanovic, Mueller, Heusser, Howarth, Holgate: "Interleukin-4, -5, and -6 and tumor necrosis factor-alpha in normal and asthmatic airways: evidence for the human mast cell as a source of these cytokines." dans: American journal of respiratory cell and molecular biology, Vol. 10, Issue 5, pp. 471-80, (1994) (PubMed).

Détails sur TNF alpha

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: Tumor Necrosis Factor-alpha

Sujet: The monoclonal antibody 52B83 reacts with tumor necrosis factor alpha (TNF-alpha). TNF-alpha is a homotrimeric 17 kDa protein, that interacts with either one of the two types of TNF-receptors, termed I and II, leading to receptor cross-linking and signal transduction. The receptors differ strongly in their intra-cellular signaling pathways. TNF-alpha was originally described as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Furthermore, TNF-alpha is found to be a central mediator in many inflammatory and immunological processes. It can be induced by various products of micro-organisms and by various cytokines leading to expression of a wide variety of cytokines. The pro-inflammatory properties of TNF-alpha play a central role in several auto-immune diseases such as rheumatoid arthritis and inhibition by neutralizing molecules have been shown to be beneficial in patients. Aliases TNF, TNF-SF2, cachectin

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome