TNF alpha anticorps

N° du produit ABIN2192066

L’anticorps Souris Monoclonal anti-TNF alpha a été validé pour IA et InhA. Il convient pour détecter TNF alpha dans des échantillons de Humain. Il y a 2+ publications disponibles.

Aperçu rapide pour TNF alpha anticorps (ABIN2192066)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TNF alpha est non-conjugé

Application: Immunoassay (IA), Inhibition Assay (InhA)

Clone: 5N

Détail du produit anti-TNF alpha anticorps

Stérilité: 0.2 μm filtered

Information d'application

Indications d'application: For neutralization of biological activity in vitro dilutions have to be made according to the amounts of -10 TNF-alpha to be inactivated. The affinity of the monoclonal antibody 5N is 5.7 x 10 M. The homologous ELISA is useful for the detection of oligomeric human TNF-alpha. It does not recognize monomeric TNF-alpha.

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.1 % bovine serum albumin.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Date de péremption: 12 months

Références pour anticorps anti-TNF alpha (ABIN2192066)

Song, Fox, Gallo, Rosenberg, Jordan, Shealy, Wagner: "Effects of 2 different anti-tumor necrosis factor-alpha agents in a primate model of subcutaneous abscess formation." dans: The Journal of infectious diseases, Vol. 185, Issue 2, pp. 204-13, (2002) (PubMed).

Petyovka, Lyach, Voitenok: "Homologous ELISA for detection of oligomeric human TNF: properties of the assay." dans: Journal of immunological methods, Vol. 186, Issue 2, pp. 161-70, (1995) (PubMed).

Détails sur TNF alpha

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: Tumor Necrosis Factor alpha

Sujet: The monoclonal antibody 5N reacts with Tumor Necrosis Factor alpha (TNF-alpha). Tumor necrosis factor-alpha (TNF-alpha), a homotrimeric 17 KD protein, is a potent mediator of inflammatory and metabolic functions. TNF-alpha was originally detected as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Further TNF-alpha has been implied as central mediator in shock induced by gram negative micro-organisms. The cytokine TNF-alpha is found to be a central mediator in many inflammatory and immunological processes: it can be induced by various products of micro-organisms and by various cytokines but it also induces on its turn the production of many cytokines. Signal transduction occurs via two types of TNF-receptors, the TNF-receptors I and II. The receptors differ strongly in their intra-cellular signaling pathways. The TNF-alpha trimer interacts with either of the two types of TNF-R leading to receptor cross-linking. The monoclonal antibody 5N cross-reacts and cross-neutralizes all monkey species TNF-alpha, except pig-tail macaque. With affinity about two orders lower it recognizes bovine and canine TNF-alpha. Aliases TNF, TNF-SF2, DIF, Cachectin

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome