BPDE-DNA anticorps

N° du produit ABIN2192143

Détail du produit anti-BPDE-DNA anticorps

Antigène: BPDE-DNA (Benzo[a]pyrenediol Epoxide Modified DNA (BPDE-DNA))

Reactivité: Chemical

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BPDE-DNA est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoassay (IA), Flow Cytometry (FACS)

Stérilité: 0.2 μm filtered

Clone: 5D11

Information d'application

Indications d'application: For immunohistochemistry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Références pour anticorps anti-BPDE-DNA (ABIN2192143)

Jurisicova, Taniuchi, Li, Shang, Antenos, Detmar, Xu, Matikainen, Benito Hernández, Nunez, Casper: "Maternal exposure to polycyclic aromatic hydrocarbons diminishes murine ovarian reserve via induction of Harakiri." dans: The Journal of clinical investigation, Vol. 117, Issue 12, pp. 3971-8, (2007) (PubMed).

Maisonnette, Simon, Hennion, Pichon: "Selective immunoclean-up followed by liquid chromatography for the monitoring of a biomarker of exposure to polycyclic aromatic hydrocarbons in urine at the ng l-1 level." dans: Journal of chromatography. A, Vol. 1120, Issue 1-2, pp. 185-93, (2006) (PubMed).

Rybicki, Rundle, Savera, Sankey, Tang: "Polycyclic aromatic hydrocarbon-DNA adducts in prostate cancer." dans: Cancer research, Vol. 64, Issue 24, pp. 8854-9, (2004) (PubMed).

Zhang, Weksler, Wang, Schwartz, Santella: "Immunohistochemical detection of polycyclic aromatic hydrocarbon-DNA damage in human blood vessels of smokers and non-smokers." dans: Atherosclerosis, Vol. 140, Issue 2, pp. 325-31, (1999) (PubMed).

Santella, Lin, Cleveland, Weinstein: "Monoclonal antibodies to DNA modified by a benzo[a]pyrene diol epoxide." dans: Carcinogenesis, Vol. 5, Issue 3, pp. 373-7, (1984) (PubMed).

Détails sur BPDE-DNA

Antigène: BPDE-DNA (Benzo[a]pyrenediol Epoxide Modified DNA (BPDE-DNA))

Abstract: BPDE-DNA Produits

Classe de substances: Chemical

Sujet: A number of chemicals, including polycyclic aromatic hydrocarbons (PAHs), have been shown to bind to DNA. This DNA damage can occur both early and late in the malignant process, thereby acting as an initiator and assisting in the progression of tumors. PAHs are released into the environment following incomplete combustion of organic materials. The most common sources of PAHs are from smoking and from consuming broiled or grilled foods. Human exposure to PAHs comes from various occupational, environmental, dietary and medicinal sources. Benzo[a]pyrene is a representative PAH. Antibodies to benzo[a]pyrenediol-epoxide modified DNA (BPDE-DNA) can be used to identify polycyclic aromatic hydrocarbon (PAH)-DNA adducts. Exposure to this group of compounds is believed to be carcinogenic. The monoclonal antibody 5D11 recognizes BPDE-I-DNA (PAH-DNA). Immunogen BPDE-I-DNA complexed with methylated BSA