For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Restrictions
For Research Use only
Buffer
PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Stockage commentaire
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Date de péremption
12 months
Oviedo, Valiño, Plasencia, Serrano, Casals, Pérez-Gil: "Quantitation of pulmonary surfactant protein SP-B in the absence or presence of phospholipids by enzyme-linked immunosorbent assay." dans: Analytical biochemistry, Vol. 293, Issue 1, pp. 78-87, (2001) (PubMed).
Pérez-Gil: "Lipid-protein interactions of hydrophobic proteins SP-B and SP-C in lung surfactant assembly and dynamics." dans: Pediatric pathology & molecular medicine, Vol. 20, Issue 6, pp. 445-69, (2001) (PubMed).
Antigène
Surfactant Protein B Cross Reactive with Human Sp-B
Sujet
The polyclonal antibody recognizes the human surfactant protein B (SP-B). There are four surfactant- specific proteins, designated surfactant protein A (SP-A), SP-B, SP-C and SP-D respectively. SP-A and SP-D are hydrophilic surfactant proteins and are members of the collectin family. SP-B and SP-C are hydrophobic surfactant proteins and may be the most appropriate indicators for the evolutionary origin of surfactant. SP-B is synthesized by the alveolar type II epithelial cells as a 40-42 kD precursor that is subsequently proteolytically processed to 7.8-8 kD. SP-B enhances the spreading and