TNF alpha anticorps

N° du produit ABIN2192175

Cet anticorps anti-TNF alpha Polyclonal Lapin (ABIN2192175) détecte spécifiquement TNF alpha dans WB, FACS, Neut, IA et ISt. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour TNF alpha anticorps (ABIN2192175)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TNF alpha est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Neutralization (Neut), Immunoassay (IA), Immunostaining (ISt)

Détail du produit anti-TNF alpha anticorps

Stérilité: 0.2 μm filtered

Information d'application

Indications d'application: For flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity dilutions have to be made according to the amounts TNF-alpha to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Date de péremption: 12 months

Détails sur TNF alpha

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: Tumor Necrosis Factor-alpha

Sujet: The antibody reacts with human natural and recombinant TNF-alpha as assessed by ELISA. The antibody inhibits the biological activity of human natural and recombinant TNF-alpha as determined with L929 and WEHI cells in a cytotoxicity assay. The antibody cross reacts with rhesus and cynomolgus natural TNF- alpha and lacks cross reactivity with human lymphotoxin.

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome