EGFR anticorps (pSer1047)

N° du produit ABIN235074

Cet anticorps anti-EGFR est un anticorps Souris Monoclonal détectant EGFR dans WB, ELISA et IFA. Adapté pour Humain.

Aperçu rapide pour EGFR anticorps (pSer1047) (ABIN235074)

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EGFR est non-conjugé

Application: Western Blotting (WB), ELISA, Indirect Immunofluorescence Assay (IFA)

Clone: 1H9

Détail du produit anti-EGFR anticorps

Épitope: pSer1047

Réactivité croisée (Details): The EGF Receptor (EFR) is activated upon stimulation with growth factors of the EGF family and autophosphorylates at multiple tyrosine residues. Serine phosphorylation of the carboxy terminal domain at Serine 1047 leads to ligand-dependent EGFR desensitization. However, if serine 1047 is phosphorylated, the neighboring tyrosine 1045 may not be phosphorylated due to sterical hindrance, thus inhibiting cbl-mediated receptor degradation. Clone 1H9 specifically recognizes EGFR phosphorylated at Serine 1047. Reacts with Human, mouse and dog.

Attributs du produit: Cell lysate of 2 x 10^6 vanadate treated HepG2 cells.

Purification: Thiophilic adsorption and size exclusion Chromatography. Cell culture

Immunogène: Synthetic phosphopeptide corresponding to AA residues surrounding Serine 1047

Isotype: IgG1

Information d'application

Indications d'application: Immunoblotting: 0.5 µg/mL for HRP/ECL detection. Recommended blocking buffer CPPT: 0.5 % (w/v) casein, 5 % (w/v) PEG 4000, 5 % (w/v) Polyvinylpyrrolidone (PVP), 0.5 % Tween 20, 10mM Tris/HCl, pH 7.4, 150mM Sodium Chloride. EGFR Transactivation Serum starved HepG2 cells were treated for 15 minutes as indicated. Whole cell lysates were separated by SDS-PAGE (20.000 cell per lane). The blot was probed with 0.5- g/mL K67190M for 1 hour at RT and developed by ECL (exp. time: 30 sec). Lane 1: control Lane 5: Sorbit Lane 2: PMA Lane 6: Anisomycin Lane 3: Forskolin Lane 7: Lonomycin Lane 4: LPA Lane 8: Taxol

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with 1 mL water for 15 minutes at room temperature.

Buffer: Lyophilized from PBS, PEG, and Sucrose, 0.09 % Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C/-80 °C

Stockage commentaire: Store lyophilized product at -20 °C. After reconstitution, aliquot and store at -80 °C. Thawed aliquots may be stored at 2-0 °C for up to 3 months.

Détails sur EGFR

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins