EGFR anticorps (pTyr1173)

N° du produit ABIN235075

Cet anticorps Souris Monoclonal détecte spécifiquement EGFR dans WB, ELISA, IP et IFA. Il présente une réactivité envers Humain.

Aperçu rapide pour EGFR anticorps (pTyr1173) (ABIN235075)

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EGFR est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Indirect Immunofluorescence Assay (IFA)

Clone: 9H2

Détail du produit anti-EGFR anticorps

Épitope: pTyr1173

Specificité: Binding of EGF to the extracellular domain of the EGF-receptor results in receptor dimerization and autophosphorylation on tyrosine residues, Y1173 being the major autophosphorylation site. Clone 9H2 specifically interacts with the 1170 - NAEpYLRV motif corresponding to the major autophosphorylation site of human EGFR. The antibody does not interact with the non-phosphorylated EGFR nor with unrelated Tyrosine-phosphorylated proteins. This phosphorylation site-specific monoclonal antibody allows the detection and quantification of activated EGFR in crude cell extracts in Immunoblot, ELISA and Biosensor assay formats without the use of P32.

Réactivité croisée (Details): Reacts with Human, mouse, and dog.

Attributs du produit: Cell lysate of pervanadate-treated HepG2 cells, Cell culture

Purification: Thiophilic adsorption and size exclusion Chromatography. Cell culture

Immunogène: Phosphopeptide conjugated to KLH

Isotype: IgG1

Information d'application

Indications d'application: Immunoblotting: 0.5 µg/mL for HRP/ECL detection. Recommended blocking buffer CPPT: 0.5 % (w/v) casein, 5 % (w/v) PEG 4000, 5 % (w/v) Polyvinylpyrrolidone (PVP), 0.5 % Tween 20, 10mM Tris/HCl, pH 7.4, 150mM Sodium Chloride. Phosphospecificity Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) A549 tumor cells were applied to SDS-PAGE (20.000 cells per lane) and transferred to a PVDF membrane. The blot was probed with 0.5- g/mL K67902M for 1 hour at RT and developed by ECL (exp. time: 30 sec). Immunoprecipitation: 1-10ug per 106 vanadate treated A431 cells. Immunocytochemistry: 1-10 µg/mL ELISA: 0.05 µg/mL Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with 1 mL water for 15 minutes at room temperature.

Buffer: Lyophilized from PBS, PEG and Sucrose, 0.09 % Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C/-80 °C

Stockage commentaire: Store lyophilized product at -20 °C. After reconstitution, aliquot and store at -80 °C. Thawed aliquots may be stored at 2-0 °C for up to 3 months.

Détails sur EGFR

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins