HBEGF anticorps (EGF Like Domain) (Biotin)

N° du produit ABIN2451994

Cet anticorps Souris Monoclonal détecte spécifiquement HBEGF dans WB, ELISA, IP, ICC et IF. Il présente une réactivité envers Humain et a été mentionné dans 4+ publications.

Aperçu rapide pour HBEGF anticorps (EGF Like Domain) (Biotin) (ABIN2451994)

Antigène: HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HBEGF est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 4G10

Détail du produit anti-HBEGF anticorps (Biotin)

Épitope: EGF Like Domain

Réactivité croisée (Details): Does not react with mouse.

Attributs du produit: biotin/IgG = 7.5

Purification: Purified

Stérilité: Sterile filtered

Immunogène: Recombinant human HB-EGF ectodomain expressed in SF21 cell

Isotype: IgG1

Information d'application

Indications d'application: 1) Western blotting: 0. 2~1 g/mL
2) Immunoprecipitation: 2 g/mL
3) Indirect immuno-fluorescence staining: 5~10 g/mL

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol

Agent conservateur: Azide free

Stock: -20 °C/-80 °C

Stockage commentaire: -20 C (For long term storage: -80 C)

Références pour anticorps anti-HBEGF (Biotin) (ABIN2451994)

Miyamoto, Hirata, Yamazaki, Kageyama, Hasuwa, Mizushima, Tanaka, Yagi, Sonoda, Kai, Kanoh, Nakano, Mekada: "Heparin-binding EGF-like growth factor is a promising target for ovarian cancer therapy." dans: Cancer research, Vol. 64, Issue 16, pp. 5720-7, (2004) (PubMed).

Prenzel, Zwick, Daub, Leserer, Abraham, Wallasch, Ullrich: "EGF receptor transactivation by G-protein-coupled receptors requires metalloproteinase cleavage of proHB-EGF." dans: Nature, Vol. 402, Issue 6764, pp. 884-8, (2000) (PubMed).

Iwamoto, Higashiyama, Mitamura, Taniguchi, Klagsbrun, Mekada et al.: "Heparin-binding EGF-like growth factor, which acts as the diphtheria toxin receptor, forms a complex with membrane protein DRAP27/CD9, which up-regulates functional receptors and diphtheria toxin ..." dans: The EMBO journal, Vol. 13, Issue 10, pp. 2322-30, (1994) (PubMed).

Higashiyama, Abraham, Miller, Fiddes, Klagsbrun: "A heparin-binding growth factor secreted by macrophage-like cells that is related to EGF." dans: Science (New York, N.Y.), Vol. 251, Issue 4996, pp. 936-9, (1991) (PubMed).

Détails sur HBEGF

Antigène: HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))

Autre désignation: HB-EGF

Sujet: Background: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is synthesized as a membrane-anchored precursor that is proteolytically cleaved to release the soluble mature growth factor, HB-EGF. The former functions as juxtacrine and the latter as paracrine growth factor. Soluble HB-EGF shows several forms in Western blotting with apparent molecular weights 19~27 kDa due to heterogeneous O-glycosylation and N-terminal truncation. HB-EGF activates EGFR and ErbB4 and promotes the development in many tissues. In human ProHB-EGF is the cellular receptor for diphtheria toxin. Non-toxic mutant of diphtheria toxin, CRM197, inhibits HB-EGF function. As HB-EGF level is elevated in most ovarian cancer, CRM197 is being tested as an anticancer drug. The hybridoma clone 4G10 was established and characterized in the laboratory of Prof. E. Mekada of Osaka University, who is a leading scientist in this field.

UniProt: Q99075

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway