MBD3 anticorps

N° du produit ABIN2458476

Détail du produit anti-MBD3 anticorps

Antigène: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MBD3 est non-conjugé

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogène: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MBD3.

Information d'application

Indications d'application: MBD3 antibody can be used for detection of MBD3 by ELISA at 1:312500. MBD3 antibody can be used for detection of MBD3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Conseil sur la manipulation: As with any antibody avoid repeat freeze-thaw cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: For short periods of storage (days) store at 4 °C. For longer periods of storage, store MBD3 antibody at -20 °C.

Détails sur MBD3

Antigène: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Autre désignation: MBD3 (MBD3 Produits)

Synonymes: anticorps xmbd3, anticorps MGC69548, anticorps MBD3, anticorps DKFZp459N1635, anticorps AI181826, anticorps AU019209, anticorps methyl-CpG binding domain protein 3 S homeolog, anticorps methyl-CpG binding domain protein 3, anticorps mbd3.S, anticorps mbd3, anticorps MBD3, anticorps Mbd3

Sujet: DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71 % and 94 % identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex.DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71 % and 94 % identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Poids moléculaire: 33 kDa

ID gène: 53615

NCBI Accession: NP_003917

UniProt: O95983

Pathways: Chromatin Binding