NEK3 anticorps

N° du produit ABIN2459259

Détail du produit anti-NEK3 anticorps

Antigène: NEK3 (NIMA related kinase 3 (NEK3))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NEK3 est non-conjugé

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogène: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NEK3.

Information d'application

Indications d'application: NEK3 antibody can be used for detection of NEK3 by ELISA at 1:1562500. NEK3 antibody can be used for detection of NEK3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Conseil sur la manipulation: As with any antibody avoid repeat freeze-thaw cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: For short periods of storage (days) store at 4 °C. For longer periods of storage, store NEK3 antibody at -20 °C.

Détails sur NEK3

Antigène: NEK3 (NIMA related kinase 3 (NEK3))

Autre désignation: NEK3 (NEK3 Produits)

Synonymes: anticorps HSPK36, anticorps ATNEK3, anticorps NIMA-RELATED KINASE3, anticorps NIMA-related kinase 3, anticorps T8M17.60, anticorps T8M17_60, anticorps NIMA related kinase 3, anticorps NIMA (never in mitosis gene a)-related expressed kinase 3, anticorps NIMA-related kinase 3 S homeolog, anticorps NIMA-related kinase 3, anticorps NEK3, anticorps Nek3, anticorps nek3.S

Sujet: NEK3 is a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. It differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase.In Aspergillus nidulans, lack of the serine/threonine kinase NimA (never in mitosis A) results in cell cycle arrest in G2, while overexpression causes the premature onset of mitotic events. The protein encoded by this gene is similar in sequence to the Aspergillus nidulans protein and may therefore play a role in mitotic regulation. However, the encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. Three transcript variants have been found for this gene, but the full-length nature of only two of them has been characterized.

Poids moléculaire: 58 kDa

ID gène: 4752

NCBI Accession: NP_002489

UniProt: P51956