CDC45 anticorps

N° du produit ABIN2459485

Détail du produit anti-CDC45 anticorps

Antigène: CDC45 (Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CDC45 est non-conjugé

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogène: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CDC45L.

Information d'application

Indications d'application: CDC45L antibody can be used for detection of CDC45L by ELISA at 1:12500. CDC45L antibody can be used for detection of CDC45L by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Conseil sur la manipulation: As with any antibody avoid repeat freeze-thaw cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: For short periods of storage (days) store at 4 °C. For longer periods of storage, store CDC45L antibody at -20 °C.

Détails sur CDC45

Antigène: CDC45 (Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45))

Autre désignation: CDC45L (CDC45 Produits)

Synonymes: anticorps cdc45l, anticorps cdc45l2, anticorps porc-pi-1, anticorps CDC45L, anticorps CDC45L2, anticorps PORC-PI-1, anticorps Cdc45l, anticorps cell division cycle 45, anticorps cell division cycle 45 S homeolog, anticorps cell division cycle 45 L homeolog, anticorps cdc45, anticorps CDC45, anticorps cdc45.S, anticorps Cdc45, anticorps cdc45.L

Sujet: CDC45L was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenlyation sites of this gene are reported. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Poids moléculaire: 65 kDa

ID gène: 8318

NCBI Accession: NP_003495

UniProt: O75419

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA