POLR2B anticorps
Aperçu rapide pour POLR2B anticorps (ABIN2460473)
Antigène
Voir toutes POLR2B AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Purification
- Antibody is purified by peptide affinity chromatography method.
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Immunogène
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLR2B.
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Indications d'application
- POLR2B antibody can be used for detection of POLR2B by ELISA at 1:1562500. POLR2B antibody can be used for detection of POLR2B by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
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Concentration
- 1 mg/mL
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Buffer
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
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Conseil sur la manipulation
- As with any antibody avoid repeat freeze-thaw cycles.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store POLR2B antibody at -20 °C.
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- POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))
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Autre désignation
- POLR2B
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Sujet
- POLR2B encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.
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Poids moléculaire
- 134 kDa
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ID gène
- 5431
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NCBI Accession
- NP_000929
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UniProt
- P30876
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Pathways
- Regulatory RNA Pathways, Réparation de l'ADN
Antigène
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