ANP32A antibody can be used for detection of ANP32A by ELISA at 1:312500. ANP32A antibody can be used for detection of ANP32A by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Conseil sur la manipulation
As with any antibody avoid repeat freeze-thaw cycles.
Stock
4 °C/-20 °C
Stockage commentaire
For short periods of storage (days) store at 4 °C. For longer periods of storage, store ANP32A antibody at -20 °C.
Antigène
PHAP1 (ANP32A)
(Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member A (ANP32A))
anticorps C15orf1, anticorps HPPCn, anticorps I1PP2A, anticorps LANP, anticorps MAPM, anticorps PHAP1, anticorps PHAPI, anticorps PP32, anticorps Anp32, anticorps W91701, anticorps pp32, anticorps Anp32a, anticorps CG5784, anticorps CT18148, anticorps CT42180, anticorps Dmel\\CG5784, anticorps anon-EST:fe3A2, anticorps wu:fj36e08, anticorps zgc:55516, anticorps ANP32D, anticorps acidic nuclear phosphoprotein 32 family member A, anticorps acidic (leucine-rich) nuclear phosphoprotein 32 family, member A, anticorps CG5784 gene product from transcript CG5784-RB, anticorps acidic nuclear phosphoprotein 32 family member A L homeolog, anticorps ANP32A, anticorps Anp32a, anticorps Mapmodulin, anticorps anp32a, anticorps anp32a.L
Sujet
ANP32A is a novel potent heat-stable inhibitor protein of protein phosphatase 2A. It may play a key role in self-renewing cell populations where it may act in the nucleus to limit their sensitivity to transformation. Being a tumor suppressor, ANP32A represses cell growth through inhibition of transcription by blocking acetylation and phosphorylation of histone H3 and initiating its proapoptotic activity.