RFC5 anticorps

N° du produit ABIN2462953

Cet anticorps Lapin Polyclonal détecte spécifiquement RFC5 dans WB, IHC et ELISA. Il présente une réactivité envers Humain et Chien.

Aperçu rapide pour RFC5 anticorps (ABIN2462953)

Antigène: RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa (RFC5))

Reactivité: Humain, Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RFC5 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-RFC5 anticorps

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogène: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RFC5.

Information d'application

Indications d'application: RFC5 antibody can be used for detection of RFC5 by ELISA at 1:62500. RFC5 antibody can be used for detection of RFC5 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Conseil sur la manipulation: As with any antibody avoid repeat freeze-thaw cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: For short periods of storage (days) store at 4 °C. For longer periods of storage, store RFC5 antibody at -20 °C.

Détails sur RFC5

Antigène: RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa (RFC5))

Autre désignation: RFC5

Sujet: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. RFC5 is the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Poids moléculaire: 38 kDa

ID gène: 5985

NCBI Accession: NP_031396

UniProt: P40937

Pathways: Telomere Maintenance, Réparation de l'ADN, DNA Replication, Synthesis of DNA