Amphiphysin anticorps (Internal Region)

N° du produit ABIN2465168

Détail du produit anti-Amphiphysin anticorps

Antigène: Amphiphysin (AMPH)

Épitope: Internal Region

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Amphiphysin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificité: This antibody is expected to recognize both reported isoforms (NP_001626.1 and NP_647477.1).

Purification: Amphiphysin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Amphiphysin antibody was raised against a 12 amino acid synthetic peptide near the internal region of Amphiphysin.

Information d'application

Indications d'application: ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 125 kDa band observed in mouse brain lysates (calculated MW of 76.3 kDa according to NP_001626.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wigge et al, Mol Biol Cell. 1997 Oct,8 (10) :2003-15., PMID: 9348539). Recommended concentration: 0.1-0.5 μg/mL. A minor band of unknown identity was also consistently observed at 45 kDa . This band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human cerebellum shows nuclear and fibrous staning. Recommended concentration, 2-4 μg/mL.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20 °C.

Détails sur Amphiphysin

Antigène: Amphiphysin (AMPH)

Autre désignation: Amphiphysin (AMPH Produits)

Synonymes: anticorps Amp, anticorps CG8604, anticorps DAMP, anticorps Damp, anticorps Dmel\\CG8604, anticorps amph, anticorps dAmph, anticorps damph, anticorps AMPH, anticorps Amph, anticorps GB16263, anticorps AMPH1, anticorps Amph1, anticorps wu:fq25h04, anticorps zgc:73193, anticorps amphiphysin, anticorps Amphiphysin, anticorps amphiphysin L homeolog, anticorps AMPH, anticorps Amph, anticorps amph, anticorps LOC409851, anticorps amph.L, anticorps LOC100343468

ID gène: 273

NCBI Accession: NP_001626, NP_647477

UniProt: P49418